Anti Nucleobindin 2/ NEFA / Nesfatin precursor - Cosmo Bio Co.,Ltd.



Anti Nucleobindin 2/ NEFA / Nesfatin precursor Antibody

NEFA, Nesfatin precursor, NUCB2

Catalog No.: BAM-73-109-EX
Size: 100UL
Price: ¥34500
antigen/source: Nucleobindin 2
host: Rabbit
Description: [Immunogen]a.a. 26-420
Purity: Serum
Application: Western Blot
Immunocytochemistry (cell)
Chromatin Immunoprecipitation
catalog info: Catalog 2012-p71
Storage: -20C
Immunogen: Mouse
Clone: NET1
Reacts with: Human
Shape: Liquid


Fig.1 Western blotting using this antibody. Distribution of nucleobindin2 and necdin in neonatal mouse organs. Homogenates of various organs from P0 mouse were separated by 10% SDS-PAGE and immunoblotted with antibody NET1 (upper panel) and anti-necdin antibody (lower panel). Nucleobindin2 immunoreactive bands (~55 kDa) were detected in all the tissues examined. Tissue levels of nucleobindin2 were high in the lung, brain, skeletal muscle, and spleen. On the other hand, necdin was enriched in the brain and skeletal muscle, and small amounts of necdin were detected in the kidney, lung, and heart.


Fig.2 Immunocytochemistry for endogenous nucleobindin2 and necdin in P19 cells. Cells were stained with antibody NET1 and anti-necdin antibody by the avidin-biotin-peroxidase complex method. Left panels, undifferentiated P19 cells (UD); right panels, enriched postmitotic neurons (PN); upper panels, nucleobindin2 immunoreactivity (Nucleobindin2); lower panels, necdin immunoreactivity (Necdin). Nucleobindin2 was localized to the cytoplasm near the nucleus in undifferentiated P19 cells, and its immunoreactivity in the cytoplasm was increased when P19 cells were induced to differentiate into neurons. On the other hand, the cytoplasm of undifferentiated cells was lightly stained for necdin, whose immunoreactivity was increased in both the cytoplasm and the nucleus of differentiated neurons.


Fig.3 Immunohistochemistry for nucleobindin2 using this antibody in neonatal mouse brain in vivo. Frozen brain sections from P0 mouse were stained with antibody NET1 by the avidin-biotin peroxidase complex method. A-C, cerebral cortex (parietal lobe). At higher magnification (B, C), fine granular immunoreactive materials are observed at both neuronal dendrites (arrows) and perikarya (asterisks) in the layer IV (arrowhead B in A) and subplate (arrowhead C in A) of the cerebral cortex. Scale bars, 100 um (A) and 10um (B and C).