Anti MAGE-D1 / Dlxin-1 / NRAGE - Cosmo Bio Co.,Ltd.



Anti MAGE-D1 / Dlxin-1 / NRAGE Antibody

melanoma-associated antigen D1, Dlxin-1, NRAGE

Catalog No.: BAM-74-112-EX
Size: 100UL
Price: ¥34500
antigen/source: MAGE-D1
host: Rabbit
Description: [Immunogen]a.a. 1-775
Purity: Serum
Application: Western Blot
catalog info: Catalog 2012-p62
Storage: -20C
Immunogen: Mouse
Clone: MD1
Reacts with: Human
Shape: Liquid


Fig.1 Western blot analysis using this antibody. Western blot analysis of necdin and MAGE-D1 in mouse embryonal tissues (ref.2). Tissue lysates (20 ug) from E18.5 embryos were separated by SDS-PAGE and immunoblotted with antibodies against necdin, MAGE-D1 (D1) and tubulin. Endogenous ~43-kDa necdin protein was detected almost exclusively in the brain and skeletal muscle whereas endogenous ~85-kDa MAGE-D1 protein was expressed in a ubiquitous manner.


Fig.2 Immunohistochemistry using this antibody (ref.2). Fluorescence immunohistochemistry for necdin and MAGE-D1 in mouse embryos. Adjacent sections of the forebrain at E12.5 embryo (upper panels) and the hind limb at E14.5 (lower panels) were stained with antibodies against necdin and MAGE-D1. The arrows point to the preplate (upper panels) and the skeletal muscles (lower panels). The arrowheads indicate the ventricular proliferative zone; V, ventricle; C, bone cavity. Necdin and MAGE-D1 were concentrated in the preplate of the forebrain at E12.5 and skeletal muscle tissues in the hind limb at E14.5. In developing neural tube, MAGE-D1 immunoreactivity was distributed in the ventricular zone as well as the marginal zone.


Fig.3 Immunocytochemistry using this antibody (ref.2). C2C12 cells were cultured under undifferentiated conditions (UD) or under differentiation conditions for 4 days (4D). Cells were fixed and triply stained for myosin heavy chain (MHC), MAGE-D1 (D1), and nuclear DNA with Hoechst33342 (Hoechst). The arrowheads point to differentiated multinucleated myocytes. MHC and MAGE-D1 were distributed predominantly in the cytosol of multinucleated differentiated C2C12 cells.