Anti MAGE-G1 / Necdin-like 2 - Cosmo Bio Co.,Ltd.



Anti MAGE-G1 / Necdin-like 2 Antibody

melanoma-associated antigen G1, Necdin-like 2

Catalog No.: BAM-74-114-EX
Size: 100UL
Price: ¥34500
antigen/source: MAGE-G1
host: Rabbit
Description: [Immunogen]a.a. 1-279
Purity: Serum
Application: Western Blot
catalog info: Catalog 2012-p62
Storage: -20C
Immunogen: Mouse
Reacts with: Human
Shape: Liquid


Fig.1 Immunoblotting of MAGE-G1 with this antibody (ref.2). E2F1, p75NTR, necdin, and MAGE-G1 (G1) were analyzed by Western blotting in whole cell lysates of P19 cells at different stages of neural differentiation. UN, undifferentiated P19 cells; RA, aggregated cells treated with retinoic acid; PN, enriched postmitotic neurons. The result revealed that P19 embryonal carcinoma cells express necdin (43 kDa), MAGE-G1 (32 kDa), E2F1 (58 kDa), and p75NTR (68 and 75 kDa) during the course of neuronal differentiation. The level of MAGE-G1 was the highest in retinoic acid-treated P19 cells.


Fig.2 Immunoaffinity purification with anti-MAGE-G1 IgG (ref.2). Dtection of endogenous complexes of MAGE-G1 with E2F1 and p75NTR.@ The lysate from retinoic acid-treated P19 cells was applied to immunoaffinity columns of anti-MAGE-G1 IgG (αG1 IgG) and preimmune IgG (Preimmune IgG). Bound proteins were immunoblotted for E2F1, p75NTR, and MAGE-G1 (G1). MAGE-G1 endogenously forms stable complexes with E2F1 and p75NTR in differentiated P19 cells.