Anti MCM7 - Cosmo Bio Co.,Ltd.



Anti MCM7 Antibody

Catalog No.: BAM-70-120EX
Size: 100UG
Price: ¥33000
antigen/source: MCM7
host: Rabbit
Label: Unlabeled
Purity: IgG fraction
Application: Western Blot
Immuno Fluorescence
Storage: -20C
Immunogen: Human
Reacts with: Human


Fig. 1. Identification of MCM7 protein in whole cell extracts of human cells by western blotting using anti-MCM7 antibody. Lane 1; SiHa cells Lane 2; C33A cells Lane3; WI38 cells All cell lines are cervical cancer derived. Samples are obtained from approximately 105 cells.


Fig. 2 Identification of MCM7 protein in whole cell extracts of human and mouse cells by western blotting using anti-MCM7 antibody.. Lane 1. Size marker proteins in kDa. Lane 2. Extract of HeLa cells untreated (-). Lane 3. Extract of HeLa cells treated with 100 nM adriamycin for 24 hr (+) Lane 4. Extract of NIH3T3 (mouse) cells. Anti-MCM7 antibody was used at 1/2,000 dilution. * Indicates the band of MCM7 protein


Fig. 3. Immunoprecipitation of MCM7 protein from crude extract of human fibroblast cell line WI38 by using anti-MCM7 antibody.. Lane 1; Immunoprecipitation with pre-immune serum Lane 2; Immnoprecipitation with anti-MCM7 antiserum. Cells were labeled with S35 methionine and MCM7 was immunoprecipitated with the anti-MCM7 antibody followed by SDS-PAGE and autoradiography


Fig. 4. Immunofluorecence staining and confocal microscopic analysis of MCM7 in G1 phase HeLa cell nucleus by using anti-MCM7 antibody after treatment with protein cross-linking reagent, DSP and chromatin extraction. The processed cells were fixed with formaldehyde before staining.