Anti DNA polymerase 1 - Cosmo Bio Co.,Ltd.



Anti DNA polymerase 1 Antibody

Catalog No.: BAM-61-012EX
Size: 100UL
Price: ¥34500
antigen/source: DNA polymerase 1
host: Rabbit
Label: Unlabeled
Purity: Serum
Application: Western Blot
Storage: -20C
Immunogen: Escherichia coli
Reacts with: Escherichia coli
Shape: Liquid


1. Western blot. (1/1,000~ 1/5,000 dilution)
2. Immunoprecipitation (assay dependent)
Not tested for other applications
 Cross reactivity 
E. coli DNA polymerase 1. Not tested in other species


Fig.1 Western blot analysis of DNA polymerase 1 in crude extract of E. coli cells by using anti-DNA polymerase 1 antibody. 1; Purified E. coli DNA polymerase 1 (10 ng) 2; Sonic lysate supernatant of E. coli strain AB1157 cells. Primary antibody was used at 1/2,000 dilution. Predicted molecular mass of DNA polymerase 1 is 103 kDa.


Fig.2 Immunoprecipitation of DNA polymerase 1 from crude lysate of E. coli with anti-DNA polymerase 1 antibody 1. Purified DNA polymerase 1 ( 20 ng), WB 2. Purified DNA polymerase 1 (10 ng), WB 3. Crude lysate of E. coli strain AB1157 (10 µg), WB 4. The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP for IP (Abcam 131368) was used.