Anti GRWD1 - Cosmo Bio Co.,Ltd.



Anti GRWD1 Antibody

Catalog No.: BAM-70-130EX
Size: 100UG
Price: ¥34500
antigen/source: GRWD1
host: Rabbit
Label: Unlabeled
Purity: Affinity Purified
Application: Western Blot
Immuno Fluorescence
Storage: -20C
Immunogen: Human
Reacts with: Human


Glutamate-rich WD repeat-containing protein 1 (GRWD1) consists of 446 amino acids with
molecular mass of 49.4 kDa. It has been found as a protein which interacts with METTL18 and CDT1 proteins.
It has been implicated in regulationof DNA replication and/or in ribosome biogenesis.
1. Western blotting (1/1,000~1/3,000 dilution)
2. Immunoprecipitation (Assay dependent)
3. Immunofluorescence staining / Immunochemistry (1/100~1/1,000 dilution)
Purified GST-GRWD1 (human, full-length) expressed in E. coli
 Cross reactivity 
Human, mouse and rat. Other species have not been tested.


Fig.1. Identication of GRWD1 proteins in whole cell lysates by western blotting with anti-GRWD1 antibody Whole cell lysates of HeLa cells untreated (-) and treated (+) with DNA damaging agent, adriamycin (Ad), and NIH3T3 cells were analyzed by western blotting with anti-GRWD1 antibody at 1/1,000 dilution. The samples were 10 µg. Second antibody was HRP-conjugated goat anti-rabbit IgG used at 1/5,000 dilution. The revelation of multiple bands indicates post-translational modification such as phosphorylation. The level of GRWD1 in the cell was not affected by DNA-damaging treatment. The identity of an additional band at ~85 kDa position other than the GRWD1 band in NIH-3T3 cell lysate is not known. The GRWD1 proteins were identified at a position higher (~55 kDa) than expected from the molecular mass of GRWD1 indicated from cDNA sequence (49.4 kDa).


Fig.2. Immunofluorescence staining of GRWD1 protein in HeLa cells. Hela cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-GRWD1 antibody was used at 1/1,000 dilution. As second antibody, goat anti-rabbit IgG conjugated with Alex488 was used at 1/5,000 dilution. As a signal enhancer, Can Get Signal Immunostain B (Toyobo, Osaka) was used according to the protocol of the supplier. Nuclei were stained with DAPI. GRWD1 protein is localized in nuclei.