Anti SPESP1 - Cosmo Bio Co.,Ltd.



Anti SPESP1 Antibody

Catalog No.: BAM-73-065-EX
Size: 100UL
Price: ¥34500
antigen/source: SPESP1
host: Rabbit
Application: Western Blot
Immuno Fluorescence
Storage: -20C
Immunogen: Mouse
Reacts with: Mouse


Fig.1 Analysis of PMIS2 protein expression in testes extracts from wild-type (+/+), and SPESP1single (+/-) and doble knockout mice by western blotting with anti-SPESP1 antibody. Testes and sperm were lyzed in lysis buffer containing 1% Triton-X100 and extracts were prepared as supernatants of lysates after centrifugation. Samples were reacted with anti-PMIS2 antibody at 1/1,000 dilution. The difference in predicted molecular mass (11 kDa) and the band position (18 kDa) of PMIS protein might be due to glycosylation.


Fig.2 Immunofluorescence staining of mouse SPESP1 with anti-SPESP1 antibody. A. Round spermatid B. Epididymal sperm As secondary antibody, Alexa Fluor 488 conjugated anti-rabbit IgG antibody was used.