記事ID ： 2758

炎症マーカーの定量キットをお求め安い価格でご提供 CRP／SAP測定ELISAキット

メーカー名：Helica Biosystems Inc.

Helica Biosystems社では、血漿、血清中の炎症マーカーCRPおよびSAPを迅速に、高感度かつ特異的に測定できるキットを販売しています。CRP測定キットはヒト、マウス、ラット、イヌ、サル、ウサギ用を、SAP測定キットはマウス用をラインアップしております。

背景

CRPとは？

CRP（C-reactive protein／C反応性タンパク質）は、肺炎双球菌の成分であるC-多糖類と反応する蛋白ということから命名されました。その構造は5種類の独立したサブユニットが非共有結合で結合した、分子量110～140kDaの環状5量体を形成しています。

CRPは肝臓で合成される血清タンパクの一種で、細菌感染や急性炎症などがあるとその濃度が上昇します。CRPレベルは炎症の兆候が見られる1時間以内に上昇し、急性期にはピークに達し、炎症回復期には減少します。それ故CRPを検出することは炎症過程の非常に有用なインジケーターとなります。

また、癌の進展に伴なってその濃度が上昇するので、腫瘍マーカーとしても注目されています。

SAPとは？

SAP（Serum Amyloid P／血清アミロイドP）は、炎症、細菌感染、および組織損傷の状態にある肝臓で産生される主要な急性期反応物質であることがマウスの実験でわかっており、長期的な体液性免疫を引き起こすために重要です。SAPの発現量は、マウス系統によって異なりますが、いずれの系統でも、インターロイキン6やリポ多糖などの外部刺激に応答して増加します。マウスSAP（mSAP）は、同一の10個のサブユニットから構成され、非共有結合的に連結した2つの五量体を形成します。

mSAPは、カルシウム依存的にエタノールアミンリン酸（phosphorylethanolamine）に結合することが知られており、それを基に本アッセイが開発されました。マウスSAPの定量化は、疾患活動性を評価する上で特に有用です。

特長

96ストリップマイクロウェルを使用したアッセイです。
経済的で使いやすいキットです。
高い特異性で動物種間での交差性がありません。
高い検出感度と再現性を実現しました。
少量サンプルからでもテスト可能です。
独自仕様のスタンダードと標識は非常に安定です。
色分けされたバイアルが便利です。
引用文献多数あります！

構成内容

CRP ELISAキット：

・ヒト、マウス、ラット、イヌ、サル用

CRP ELISA マイクロプレート
HRP標識抗CRP-IgG
スタンダード
洗浄バッファー
TMB基質
停止液

・ウサギ用

CRP ELISA マイクロプレート
ビオチン標識抗CRP-IgG
アビジンペルオキシダーゼコンジュゲート
スタンダード
洗浄バッファー
TMB基質
停止液

マウスSAP ELISAキット：

SAP ELISAマイクロプレート
マウスSAPスタンダード
ウサギ抗マウスSAP抗体
HRP標識抗ウサギIgG抗体
TMB基質
停止液
洗浄バッファー

その他

■ 測定範囲
測定対象	対象動物種	測定範囲	品番
CRP	Human	0.12-10 ng/mL	961CRP01H-96
	Mouse	1.56-25 ng/mL	971CRP01M-96
	Rat	2.5-133 ng/mL	951CRP01R-96
	Canine	3.6-40 ng/mL	931CRP01D-96
	Primate	0.12 - 10 ng/mL	911CRP01P-96
	Rabbit	1.56 - 25 ng/mL	921CRP01L-96
SAP	Mouse	5.12-200 ng/mL	151SAP01M-96

使用例

図1. SAP測定ELISAキット（品番：151SAP01M-96）のスタンダードカーブ例

CRP（C反応性タンパク質）測定ELISAキット

■ ヒト

品名	メーカー	品番	包装	希望販売価格
C-Reactive Protein ELISA, Human	HLC	961CRP01H-96	96 TEST [96 well]	販売終了

■ マウス

品名	メーカー	品番	包装	希望販売価格
C-Reactive Protein ELISA, Mouse	HLC	971CRP01M-96	1 KIT [96 well]	販売終了

■ ラット

品名	メーカー	品番	包装	希望販売価格
C-Reactive Protein ELISA, Rat	HLC	951CRP01R-96	1 KIT [96 well]	販売終了

■ イヌ

品名	メーカー	品番	包装	希望販売価格
C-Reactive Protein ELISA, Canine	HLC	931CRP01D-96	1 KIT [96 well]	販売終了

■ サル

品名	メーカー	品番	包装	希望販売価格
C-Reactive Protein ELISA, Primates	HLC	911CRP01P-96	96 TEST [96 well]	販売終了

■ ウサギ

品名	メーカー	品番	包装	希望販売価格
C-Reactive Protein ELISA, Rabbit	HLC	921CRP01L-96	96 TEST [96 well]	販売終了

Serum Amyloid P 測定ELISAキット

■ マウス

品名	メーカー	品番	包装	希望販売価格
Serum Amyloid P (SAP) ELISA, Mouse	HLC	151SAP01M-96	96 TEST	販売終了

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