■ CD163 モノクローナル抗体(品番:65169-1-Ig)
図1. CD163抗体とアイソタイプコントロール抗体を用いた細胞表面マーカー解析
1X10^6 human peripheral blood monocytes were surface stained with 0.5 ug Anti-Human CD163 (65169-1-Ig, Clone: GHI/61) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Samples were not fixed.
■ CD163 モノクローナル抗体(品番:PE-65169)
図1. CD163抗体とCD14抗体を用いた細胞表面マーカー解析
0.1 mL human peripheral blood were surface stained with 10 ul FITC-Anti-Human CD14 (PE-65056, Clone: UCHM-1) and 10 ul PE-Anti-Human CD163 (PE-65169, Clone: GHI/61). Sample was then treated with red blood cell lysis buffer.図2. アイソタイプコントロール抗体とCD14抗体を用いた細胞表面マーカー解析
0.1 mL human peripheral blood were surface stained with 10 ul FITC-Anti-Human CD14 (PE-65056, Clone: UCHM-1) and PE-Mouse IgG1 isotype control antibody. Sample was then treated with red blood cell lysis buffer.
■ CD163 モノクローナル抗体(品番:APC-65169)
図1. CD163抗体とCD14抗体を用いた細胞表面マーカー解析
100 ul human peripheral blood were surface stained with 10 ul FITC Anti-Human CD14 (FITC-65056, Clone: UCHM-1), and 10 ul APC Anti-Human CD163 (APC-65169, Clone: GHI/61) or APC Mouse IgG1 isotype control. Samples were then treated with red blood cell lysis buffer and were gated for CD14+ monocytes for analysis of CD163 staining. Samples were not fixed.
■ CD163 モノクローナル抗体(品番:CL488-65169)
図1. CD163抗体とCD14抗体を用いた細胞表面マーカー解析
100 ul human peripheral blood were surface stained with 10 ul PE-Anti-Human CD14 (PE-65056, Clone: UCHM-1), and 5 ul CoraLite®488-conjugated Anti-Human CD163 (CL488-65169, Clone: GHI/61) or CoraLite®488-conjugated Mouse IgG1 isotype control. Samples were then treated with red blood cell lysis buffer and were gated for CD14+ monocytes for analysis of CD163 staining.
■ CD163 モノクローナル抗体(品番:CL647-65169)
図1. CD163抗体とCD14抗体を用いた細胞表面マーカー解析
100 ul human peripheral blood were surface stained with 10 ul FITC-Anti-Human CD14 (FITC-65056, Clone: UCHM-1), and 5 ul CoraLite®647-conjugated Anti-Human CD163 (CL647-65169, Clone: GHI/61) or CoraLite®647-conjugated Mouse IgG1 isotype control. Samples were then treated with red blood cell lysis buffer and were gated for CD14+ monocytes for analysis of CD163 staining. Samples were not fixed.