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Complete Xeno-Free Culture System for the Isolation , Expansion and Differentiation of Human Mesenchymal Stem Cells - towards Clinical Applications
―臨床応用に向けたヒト間葉系幹細胞の単離・増殖培養・分化のための完全なXeno-Free 細胞培養システム―
講演者 ： David Fiorentini, VP for Scientific Affairs of Biological Industries ltd.
座長 ： 王 英正 先生（岡山大学病院 新医療研究開発センター）
会場 ： パシフィコ横浜 会議センター 419号室（第6会場）
日時 ： 2015年3月21日（土）12:00〜12:50
要旨
ヒト間葉系幹細胞(hMSC)は骨髄、脂肪、Wharton's jellyといった様々なヒト組織から単離できる組織幹細胞で、骨、軟骨や脂肪細胞への分化能を持つため、再生医療分野で非常に注目されている細胞の一つです。 しかしながら、hMSCは in vitro で増殖培養される際、培地成分や培養条件により大きく影響を受けるため、その培養環境の品質は非常に重要となります。
本セミナーでは、MSC Nutristem? XF培地および関連試薬で構築される、hMSCの単離・増殖培養および分化に最適化したコンプリートXeno-Free培養系を用いた、様々な組織由来のhMSCの品質評価及び分化能についてご紹介致します。
Human mesenchymal stem cells (hMSCs) are multipotent adult stem cells that can be isolated from variety of human tissues such as bone marrow, adipose, placenta, cord blood and Wharton jelly. hMSC have the ability to differentiate into bone, cartilage and fat cells and are the subject of many clinical trials for a range of regenerative and inflammatory diseases.
hMSC have advantages over other stem cell types due to the broad variety of their tissue sources, since they are immuno-privileged, and for their ability to specifically migrate to the site of injury in vivo.
In most clinical applications hMSC are expanded in vitro before use. The quality of the culture media and auxiliary solutions is particularly crucial with regard to therapeutic applications, since hMSC properties can be significantly affected by medium components and culture conditions.
To date, most of the common culture media for expansion and differentiation of hMSC, as well as auxiliary solutions (for attachment, cryopreservation and dissociation) are supplemented with serum or other xenogenic compounds. A defined serum-free (SF), xeno-free (XF) culture system optimized for hMSC isolation, expansion and differentiation would greatly facilitate the development of robust, clinically acceptable cell culture process for quality-assured cells.
The present study evaluate an innovative xeno-free culture system, comprising of MSC NutriStem? XF culture medium and the required auxiliary solutions to support efficient isolation, expansion, cryopreservation and differentiation of hMSC towards clinical applications.
Results show that the XF culture system for hMSC efficiently supports initial isolation and optimal expansion of hMSC from various sources, while maintaining hMSC features: typical fibroblast-like cell morphology, phenotypic surface marker profile, differentiation capacity, self-renewal potential, and genetic stability.
In addition, the xeno-free differentiation media can efficiently generate mature adipocytes, osteoblasts and chondrocytes using hMSC from variety of sources.
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