ヒト、マウス、ラット、ウサギなど動物種毎に最適密度に調製済み！！ Lympholyte® リンパ球 (PBMC) / Lympholyte®-poly 顆粒球 分離溶液

マウス/ ラット/ ウサギ/ ヒトなどの哺乳類細胞から、生きたリンパ球もしくは顆粒球を遠心により分離するための試薬です。リンパ球を高い収率で回収し、細胞毒性、FACS、in vivo/in vitro 等の実験に使用できます。全ての商品は、滅菌済み、各動物種ごとに最適な密度に調製済の液状品です。

免疫細胞濃縮カラム／キット／溶液

• Lympholyte® リンパ球分離溶液
• Lympholyte®-PURE マウスの様々な免疫細胞集団から特定の細胞を濃縮するキット
• フローサイトメトリー用抗体

• リンパ組織からリンパ球（PBMC)を分離
• 以下の細胞群から死細胞・細胞破片を除去
  • 細胞培養後抗体及び補体処理したリンパ球懸濁液
  • 連続した細胞毒性実験後の細胞群（B 細胞枯渇など）
  • 各種クローナル細胞及びハイブリドーマ細胞種
• 各種クローナル細胞及びハイブリドーマ細胞から赤血球、死細胞、細胞破片を除去（Lympholyte®-M、Lympholyte®-R）
• マウス核上皮細胞の分離（Lympholyte®-M）
• 骨髄サンプルから単核球を分離（Lympholye®-H）

A またはB
  A： 細胞懸濁液又は全血サンプルをLympholyte® の上に層状に重ねます
  （2層を混合しないように注意）
  B： パスツールピペットを用いて細胞懸濁液又は全血サンプルの下層にLympholyte® をゆっくり加えます
C：室温で遠心分離します
D：パスツールピペットでリンパ球層を新しい遠心チューブに移し、洗浄します

Lympholyte®-H
Spinelli E, Caporale R, Buchi F, Masala E, et al. (2012) Distinct Signal Transduction Abnormalities and Erythropoietin Response in Bone Marrow Hematopoietic Cell Subpopulations of Myelodysplastic Syndrome Patients. Clin Cancer Res. 18 (11):3079-89.
Colasanti T, Alessandri C, Capozzi A, et al. (2012) Autoantibodies specific to a peptide of β2-glycoprotein I cross-react with TLR4 inducing a pro-inflammatory phenotype in endothelial cells and monocytes.Blood. 120 (16):3360-70.
Pievani A, Belussi C, Klein C, Rambaldi A, Golay J, Introna M. (2011) Enhanced killing of human B-cell lymphoma targets by combined use of cytokine-induced killer cell (CIK) cultures and anti-CD20 antibodies. Blood. 117 (2):510-8.
Kuznik A, Bencina M, Svajger U, Jeras M, Rozman B, Jerala R. (2011) Mechanism of Endosomal TLR Inhibition by Antimalarial Drugs and Imidazoquinolines. J Immunol. 186 (8):4794-804.

Lympholyte®-M
Garaycoechea JI, Crossan GP, Langevin F, et al. (2012) Genotoxic consequences of endogenous aldehydes on mouse haematopoietic stem cellfunction. Nature. 489 (7417):571-5.
Pulido J, Kottke T, Thompson J, et al. (2012) Using virally expressed melanoma cDNA libraries to identify tumor-associated antigens that cure melanoma. Nat Biotechnol. 18; 30(4):337-43.
Revilla-I-Domingo R, Bilic I, Vilagos B, Tagoh H, et al. (2012) The B-cell identity factor Pax5 regulates distinct transcriptional programmes in early and late B lymphopoiesis. EMBO J. 31 (14):3130-46.
Sato K, Misawa N, Nie C, Satou Y, et al. (2011) A novel animal model of Epstein-Barr virus associated hemophagocytic lymphohistiocytosis in humanized mice. Blood. 117 (21):5663-73.
Soda Y, Marumoto T, Friedmann-Morvinski D, et al. (2011) Transdifferentiation of glioblastoma cells into vascular endothelial cells. PNAS. 108 (11):4274-80.
Nouze C, Pasquet L, van Meerwijk JP. (2011) In Vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection. Methods Mol Biol. 707:187-96.
Gray EE, Suzuki K, Cyster JG. (2011) Identification of a motile IL-17 producing γδ T cell population in the dermis. J. Immunol. 186 (11):6091-5.
Tessmer MS, Reilly EC, Brossay L. (2011) Salivary Gland NK Cells Are Phenotypically and Functionally Unique. PLoS Pathog. 7 (1):e1001254.
Becker MR, Choi YS, Millar SE, Udey MC. (2011) Wnt Signaling Influences the Development of Murine Epidermal Langerhans Cells. J Invest Dermatol. 131 (9):1861-8.

Lympholyte®-Mammal
Hoggatt J, Mohammad KS, Singh P, Hoggatt AF, et al. (2013) Differential stem- and progenitor-cell trafficking by prostaglandin E2. Nature. 495 (7441):365-9.
Lin WH, Kouyos RD, Adams RJ, Grenfell BT, Griffin DE. (2012) Prolonged persistence of measles virus RNA is characteristic of primary infection dynamics. PNAS. 109 (37):14989-94.
Lin WH, Griffin DE, Rota PA, Papania M, et al. (2011) Successful respiratory immunization with dry powder live-attenuated measles virus vaccine in rhesus macaques. PNAS. 108 (7):2987-92.
Hu S, Yang K, Yang J, Li M, Xiong N. (2011) Critical roles of chemokine receptor CCR10 in regulating memory IgA responses in intestines. PNAS. 108 (45):E1035-44.

Lympholyte®-Rabbit
Cameron CM, Barrett JW, Liu L, et al (2005) Myxoma virus M141R expresses a viral CD200 (vOX-2) that is responsible for down-regulation ofmacrophage and T-cell activation in vivo. J Virol. 79 (10):6052-67.

Lympholyte®-R
Bhalli JA, Shaddock JG, Pearce MG, et al. (2011) Report on stage III Pig-a mutation assays using benzo[a]pyrene. Environ Mol Mutagen. 52 (9):731-7.
Zhang ZX, Ma Y, Wang H, et al. (2006) Double-negative T cells, activated by xenoantigen, lyse autologous B and T cells using a perforin/granzyme-dependent, Fas-Fas ligand-independent pathway. J Immunol. 177 (10):6920-9.

Lympholyte®-poly
Swystun LL, Mukherjee S, Liaw PC. (2011) Breast cancer chemotherapy induces the release of cell-free DNA, a novel procoagulant stimulus. J Thromb Haemost. 9 (11):2313-21.
Marquez-Curtis L, Jalili A, Deiteren K, et al. (2008) Carboxypeptidase M expressed by human bone marrow cells cleaves the C-terminal lysine of stromal cell-derived factor-1alpha: another player in hematopoietic stem/progenitor cell mobilization Stem Cells. 26 (5):1211-20.

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