記事ID ： 2771

高血圧の研究に有用 14,15-DHET 測定ELISAキット

メーカー名：DETROIT R&D, INC.

14,15-DHET 測定ELISAキットは、高血圧に関連する物質であるDHETを定量するためのキットです。

14,15-DHET 測定ELISAキット

背景

14,15-DHETは、シトクロムP450 (CYPs) 2Cおよび2Jのアラキドン酸エポキシゲナーゼ活性によって生成されたEETが可溶性エポキシド加水分解酵素 (sEH) により触媒された代謝産物です。

本キットはHRPシステムを使った競合ELISAキットで、生物学的サンプル (組織、血漿、尿) および細胞培養培地中の 14,15-DHETレベルを決定します。本キットにより、高血圧や脳損傷、脳卒中と 14, 15-DHETレベルに、また癌細胞の転移表現型、インスリン抵抗性と EETレベルに強い正相関が確認されています。
現在、次世代の高血圧治療薬の開発がいくつかの研究所で行われています。

各キットの96wellプレートは、最大24サンプルをtriplicateで測定することができます。

特長

競合ELISA
交差性：ヒト
テストサンプル：血清、血漿、細胞・組織抽出物
測定範囲：10 ～ 1,000,000 pg/mL
サンプルの抽出、精製方法も掲載された丁寧なプロトコール

構成内容

14,15-DHET ELISA プレート
14,15-DHET スタンダード
14,15-DHET-HRP コンジュゲート
サンプル希釈バッファー
HRPバッファー
洗浄バッファー
TMB基質

14,15-EET/DHET Hypertension ELISAキットと14,15-DHET Hypertension ELISAキットの違い

両ELISAキットの構成品は全く同じで、違いはサンプルの調整方法です。14,15-EET/DHET HypertensionELISAキットのプロトコールには、EETを測定するためのサンプル調製方法が掲載されています。

使用例

左図：高血圧自然発症ラットにおける尿中14,15-DHET濃度の上昇
右図：妊娠誘発性高血圧（子癇前症）女性の尿中14,15-DHET濃度は、健常妊婦と比較して有意差があった（p<0.02）。

使用文献

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14,15-DHET 測定ELISAキット

品名	メーカー	品番	包装	希望販売価格
14,15-DHET Hypertension ELISA kit (Goat)	DRD	DH1	1 KIT [1*96 well]	￥118,000
14,15-EET/DHET Hypertension ELISA kit (Goat)	DRD	DH2	1 KIT [1*96 well]	￥118,000

14,15-DHET 測定ELISAキット with Removeable Strips

品名	メーカー	品番	包装	希望販売価格
14,15-DHET Hypertension ELISA Kit with Removeable Strips (Goat)	DRD	DH1R	1 KIT	￥133,000

［関連］その他高血圧研究用試薬

品名	メーカー	品番	包装	希望販売価格
20-HETE Hypertension ELISA kit (Goat)	DRD	20H1	1 KIT [1*96 well]	￥118,000
8-isoprostane oxidative stress ELISA kit (Goat)	DRD	8ISO1	1 KIT [1*96 well]	￥118,000
11,12-DHET Immunoassay kit (Goat)	DRD	DH4	1 KIT [1*96 well]	￥118,000
(1-kit )11,12-EET/DHET Immunoassay kit (Goat)	DRD	DH5	1 KIT [1*96 well]	￥118,000