このページを印刷する
TNF-α ELISA キットは、ヒト・マウス・ラットの TNF-α を比色法により定量的に測定できる ELISA キットです。
背景
TNF‐α（Tumor Necrosis Factor-α：腫瘍壊死因子-α）とは、17.5kDaの157個のアミノ酸からなる、様々な腫瘍細胞および標的細胞に対して細胞毒性作用を持つサイトカインです。TNF-αは関節リウマチ患者の滑液から検出されていることから、炎症誘発性を示すことが示唆されています。TNF‐αは免疫調節の主要なメディエーターとして知られています。TNF-αの生合成は厳密に制御されており、その分泌量は休止期の細胞では少なく、活性化細胞では多くなります。
特長
- 高感度測定
- プレコートプレート入り
- 定量的な測定が可能
仕様
| 交差性 | サンプルタイプ | 感度 | 測定範囲 | 品番 |
|---|---|---|---|---|
| Human | 細胞培養上清、血漿、血清 | 8.43 pg/ml | 15.63 - 1,000 pg/ml | ADI-900-099 |
| Mouse | 細胞培養上清、血清、血漿＊、組織ホモジネート＊ | 3.9 pg/ml | 31.25 - 2,000 pg/ml | ADI-900-047 |
| Rat | 細胞培養上清 | 12 pg/ml (アッセイバッファー) 26.7 pg/ml (10 %FBS入り培養培地) |
31.3 - 2,000 pg/ml | ADI-900-086A |
＊文献に掲載されているサンプルタイプであり、ENZ社では確認していません。
使用例
図1 スタンダードカーブ
TNF-α ELISA キット
| 品名 | メーカー | 品番 | 包装 | 希望販売価格 |
|---|---|---|---|---|
TNF-α, ELISA kit, Human |
ENZ | ADI-900-099 | 96 WELL |
￥129,000 |
| TNF-α, ELISA kit, Mouse |
ENZ | ADI-900-047 | 96 WELL |
￥129,000 |
| TNF-α, ELISA kit, Rat |
ENZ | ADI-900-086A | 96 WELL |
お問い合わせ |
【関連商品】
| 品名 | メーカー | 品番 | 包装 | 希望販売価格 |
|---|---|---|---|---|
| TNF-α, Human |
ENZ | ADI-908-066-010 | 10 UG |
￥38,000 |
製品使用文献
■ヒト用（品番：ADI-900-099）
- Progranulin concentration in relation to bone mineral density among obese individuals: A. Milajerdi, et al.; Arch. Endocrinol. Metab. 62, 179 (2018), Application(s): EIA w/ human serum
- Retinal protection and distribution of curcumin in vitro and in vivo: C.B.M. Platania, et al.; Front. Pharmacol. 9, 670 (2018), Application(s): ELISA using culture supernatants
- Effects of Erythrocyte Membrane Polyunsaturated Fatty Acids in Overweight, Obese, and Morbidly Obese Korean Women: H.N. Choi, et al.; J. Cancer Prev. 22, 182 (2017), Application(s): Human serum
4.Evaluation of HIV-DNA and inflammatory markers in HIV-infected individuals with different viral load patterns: F. Falasca, et al.; BMC Infect. Dis. 17, 581 (2017) - Evaluation of HIV-DNA and inflammatory markers in HIV-infected individuals with different viral load patterns: F. Falasca, et al.; BMC Infect. Dis. 17, 581 (2017)
- Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Application(s): ELISA (to find proinflammatory cytokine production)
- The Effect of Onion Peel Extract on Inflammatory Mediators in Korean Overweight and Obese Women: K.A. Kim & J.E. Yim; Clin. Nutr. Res. 5, 261 (2016), Application(s): Plasma samples
- Association between obesity-related adipokines and colorectal cancer: A case-control study and meta-analysis: R.K. Joshi, et al.; World J. Gastroenterol. 20, 7941 (2014), Application(s): Quantification of TNF-α in serum samples
- Genetic damage in patients with chronic kidney disease, peritoneal dialysis and haemodialysis: a comparative study: A. Rangel-Lopez, et al.; Mutagenesis 28, 219 (2013), Application(s): ELISA using human serum
- Effects of red orange juice intake on endothelial function and inflammatory markers in adult subjects with increased cardiovascular risk: S. Buscemi, et al.; Am. J. Clin. Nutr. 95, 1089 (2012), Application(s): Levels of IL-6 in human serum
■マウス用（品番：ADI-900-047）
- Hepatic leukocyte immunoglobulin‐like receptor B4 (LILRB4) attenuates nonalcoholic fatty liver disease via SHP1‐TRAF6 pathway: Y. Lu, et al.; Hepatology 67, 1303 (2018), Application(s): ELISA using mouse serum
- In vivo effects of the NLRP1/NLRP3 inflammasome pathway on latent respiratory virus infection: Y.H. Li, et al.; Int. J. Mol. Med. 41, 3620 (2018), Application(s): ELISA using mouse serum
- Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Application(s): ELISA using culture supernatants
- Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum. 62, 732 (2010), Application(s): EIA using mouse serum and cell culture supernatant
- Mitogen-activated protein kinase phosphatase-1 deficiency decreases atherosclerosis in apolipoprotein E null mice by reducing monocyte chemoattractant protein-1 levels: S. Imaizumi, et al.; Mol. Genet. Metab. 101, 66 (2010), Application(s): EIA using mouse serum
- Acid sphingomyelinase involvement in tumor necrosis factor α-regulated vascular and steroid disruption during luteolysis in vivo: L.E. Henkes, et al. ; PNAS 105, 7670 (2008), Application(s): EIA using mouse tissue (ref. 1)
- Immunosuppressive Effect of Isopropanol: Down-Regulation of Cytokine Production Results from the Alteration of Discrete Transcriptional Pathways in Activated Lymphocytes: O. Desy, et al. ; J. Immunol. 181, 2348 (2008), Application(s): EIA using mouse serum
■ラット用（品番：ADI-900-086A）
- Long-term feeding of red algae (Gelidium amansii) ameliorates glucose and lipid metabolism in a high fructose diet-impaired glucose tolerance rat model: H.C. Liu, et al.; J. Food Drug Anal. 25, 543 (2017), Application(s): Determination of tumor necrosis factor-α, rat plasma
- Therapeutic role of ursolic acid on ameliorating hepatic steatosis and improving metabolic disorders in high-fat diet-induced non-alcoholic fatty liver disease rats: S. Li, et al.; PLoS One 9, e86724 (2014), Application(s): ELISA using rat serum
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用としては使用しないように、十分ご注意ください。
※ 表示価格について
- 「TNF-α ELISA キット」は、下記のカテゴリーに属しています。
中身を見る
中身を見る
中身を見る
中身を見る