記事ID ： 16439

緑色蛍光色素 CF®488A Dye

メーカー名：Biotium, Inc.

CF®488A は、最適励起波長 488 nm の緑色蛍光色素です。一般的な検出条件下では、CF®488Aは既存の競合色素と少なくとも同等の蛍光強度を示し、抗体との複合体の生物学的な特異性が高いことが主な利点として挙げられます。

既存の競合色素は多数の負電荷を持ち、タンパク質の等電点に大きく影響するため、タンパク質複合体の特異性が変わります。CF®488A は電荷が非常に低いため、本色素で標識した抗体は、高いS/N比を示します。

また、蛍光極大波長が、既存の競合色素と比較して約 10 nm、FITC（または FAM）と比較して約 15 nm 短いため、マルチカラー検出の際に、赤色チャネルでの蛍光のスピルオーバーが少ないことも利点の一つです。

Biotium社 CF® Dye 選択ガイド

特長

特異性の高い抗体複合体を形成し、赤色チャネルでの蛍光のスピルオーバーが少ない
優れた光安定性
水溶性が高く、pH非感受性

仕様

極大吸収/蛍光波長: 490/515 nm
吸光係数: 70,000
分子量: ～910
励起光源: 488 nm
スペクトル類似色素: Alexa Fluor® 488, DyLight™488, FITC, FAM, Cy®2

図1 PBS中でのCF488A標識ヤギ抗マウス抗体の吸収、蛍光スペクトル

使用例

図2 ウサギ抗フィブロネクチン抗体と、既存競合色素2種類あるいはCF488Aの各々で標識したヤギ抗マウス抗体で染色したヒト心臓の凍結切片。
提供元：Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim, Hessen, Germany.

Mix-n-Stain™ CF® Dye 抗体標識キット

品名	メーカー	品番	包装	希望販売価格
Mix-n-Stain^TM CF^(R)488A Antibody Labeling Kit, CF^TM 488A	BTI	92273	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)488A Antibody Labeling Kit, CF^TM 488A	BTI	92253	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)488A Antibody Labeling Kit, CF^TM 488A	BTI	92233	1 KIT [1x(50-100μg)labeling]	￥36,000

Mix-n-Stain™ CF® Dye 低分子リガンド標識キット

品名	メーカー	品番	包装	希望販売価格
Mix-n-Stain^TM CF^(R)488A Small Ligand Labeling Kit, CF^TM 488A	BTI	92350	1 KIT	￥47,000

CF® Dye 反応性蛍光色素

品名	メーカー	品番	包装	希望販売価格
CF^(R)488A Amine, CF^TM 488A	BTI	92037	1 MG	￥59,000
CF^(R)488A, Aminooxy, CF^TM 488A	BTI	92051	1 MG	￥120,000
CF^(R)488A Hydrazide, CF^TM 488A	BTI	92152	1 MG	￥47,000
CF^(R)488A Maleimide, CF^TM 488A	BTI	92022	1 UMOL	￥63,000
CF^(R)488A, Succinimidyl Ester, CF^TM 488A	BTI	92120	1 UMOL	￥69,000
CF^(R)488A Biocytin, CF^TM 488A	BTI	92004	0.5 MG	￥85,000
CF^(R)488A BCN, CF^TM 488A	BTI	92075	0.5 MG	￥94,000
CF^(R)488A Azide, CF^TM 488A	BTI	92080	0.5 MG	￥77,000
CF^(R)488A Alkyne, CF^TM 488A	BTI	92086	0.5 MG	￥77,000
CF^(R)488A MTS, CF^TM 488A	BTI	92097	1 MG	￥49,000
CF^(R)488A Tyramide, CF^TM 488A	BTI	92171	0.5 MG	￥77,000
CF^(R)488A Picolyl Azide, CF^TM 488A	BTI	92187	0.5 MG	￥80,000

CF® Dye タンパク質標識キット

品名	メーカー	品番	包装	希望販売価格
CF^(R)488A Protein Labeling Kit, CF^TM 488A	BTI	92213	1 KIT [3x(1mg) labelings]	￥112,000

CF® Dye 標識体

品名	メーカー	品番	包装	希望販売価格
Annexin V, CF^TM 488	BTI	29005	0.5 ML [50 ug/mL]	￥73,000
Alpha-Bungarotoxin, CF^TM 488A	BTI	00005	500 UG	￥123,000
Serum albumin, Bovine, CF^TM 488A	BTI	20289	5*1 MG	￥52,000
Concanavalin A, CF^TM 488A	BTI	29016	5*1 MG [1mg x 5 vials]	￥44,000
CF^(R)488A-dUTP, CF^TM 488A	BTI	40008	25 NMOL	￥98,000
CF^(R)488A-dCTP, CF^TM 488A	BTI	40067	25 NMOL	￥98,000
Phalloidin, CF^TM 488A	BTI	00042	300 UNIT	￥113,000
Phalloidin, CF^TM 488A	BTI	00042-T	50 UNIT	￥24,000
Streptavidin, CF^TM 488A	BTI	29034	1 MG	￥51,000
Wheat Germ Agglutinin, CF^TM 488A	BTI	29022-1	1 MG	￥20,000
Wheat Germ Agglutinin, CF^TM 488A	BTI	29022	5*1 MG	￥85,000
cAMP, CF^TM 488A	BTI	00036	100 UG	￥32,000
Cholera Toxin B, CF^TM 488A	BTI	00070	100 UG	￥60,000
Transferrin, Human, CF^TM 488A	BTI	00081	1 MG	￥26,000
CF^(R)488A Lectin PNA, Peanut, CF^TM 488A	BTI	29060	1 MG	￥38,000
CF^(R)488A-Dextran 10,000 MW, Anionic and Fixable, CF^TM 488A	BTI	80110	1 MG	￥25,000

CF® Dye 標識抗体

二次抗体

品名	メーカー	品番	包装	希望販売価格
Anti IgG(H+L), Goat (Chicken) CF^TM 488A	BTI	20225	0.5 ML [2 mg/mL]	￥47,000
Anti IgG(H+L), Goat (Chicken) CF^TM 488A	BTI	20225-1	50 UL [2 mg/mL]	￥20,000
Anti IgG(H+L), Goat (Chicken) CF^TM 488A	BTI	20225-1MG	1 MG	￥49,000
Anti IgG(H+L), Mouse (Chicken) CF^TM 488A	BTI	20208	0.5 ML [2 mg/mL]	￥47,000
Anti IgG(H+L), Mouse (Chicken) CF^TM 488A	BTI	20208-1	50 UL [2 mg/mL]	￥19,000
Anti IgG(H+L), Mouse (Chicken) CF^TM 488A	BTI	20208-1MG	1 MG	￥49,000
Anti IgG(H+L), Rabbit (Chicken) CF^TM 488A	BTI	20209	0.5 ML [2 mg/mL]	￥47,000
Anti IgG(H+L), Rabbit (Chicken) CF^TM 488A	BTI	20209-1	50 UL [2 mg/mL]	￥19,000
Anti IgG(H+L), Rabbit (Chicken) CF^TM 488A	BTI	20209-1MG	1 MG	￥49,000
Anti IgG(H+L), Guinea Pig (Goat) CF^TM 488A	BTI	20017	0.5 ML [2 mg/mL]	￥47,000
Anti IgG(H+L), Guinea Pig (Goat) CF^TM 488A	BTI	20017-1	50 UL [2 mg/mL]	￥19,000
Anti IgG(H+L), Guinea Pig (Goat) CF^TM 488A	BTI	20017-1MG	1 MG	￥49,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20010	0.5 ML [2 mg/mL]	￥47,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20010-1	50 UL [2 mg/mL]	￥19,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20010-1MG	1 MG	￥49,000
Anti IgG(H+L), Rabbit (Goat) CF^TM 488A	BTI	20012	0.5 ML [2 mg/mL]	￥47,000
Anti IgG(H+L), Rabbit (Goat) CF^TM 488A	BTI	20012-1	50 UL [2 mg/mL]	￥19,000
Anti IgG(H+L), Rabbit (Goat) CF^TM 488A	BTI	20012-1MG	1 MG	￥49,000
Anti IgG(H+L), Porcine (Goat) CF^TM 488A	BTI	20028	0.5 ML [2 mg/mL]	￥47,000
Anti IgG(H+L), Porcine (Goat) CF^TM 488A	BTI	20028-1	50 UL [2 mg/mL]	￥19,000
Anti IgG(H+L), Porcine (Goat) CF^TM 488A	BTI	20028-1MG	1 MG	￥49,000
Anti IgY(IgG)(H+L), Chicken (Rabbit) CF^TM 488A	BTI	20079	0.5 ML [2 mg/mL]	￥47,000
Anti IgY(IgG)(H+L), Chicken (Rabbit) CF^TM 488A	BTI	20079-1	50 UL [2 mg/mL]	￥19,000
Anti IgY(IgG)(H+L), Chicken (Rabbit) CF^TM 488A	BTI	20079-1MG	1 MG	￥49,000
Anti IgG(H+L), Goat (Rabbit) CF^TM 488A	BTI	20021	0.5 ML [2 mg/mL]	￥47,000
Anti IgG(H+L), Goat (Rabbit) CF^TM 488A	BTI	20021-1	50 UL [2 mg/mL]	￥19,000
Anti IgG(H+L), Goat (Rabbit) CF^TM 488A	BTI	20021-1MG	1 MG	￥49,000
Anti IgG(H+L), F(ab')2, Mouse (Goat) CF^TM 488A	BTI	20011	0.25 ML [2 mg/mL]	￥37,000
Anti IgG(H+L), F(ab')2, Mouse (Goat) CF^TM 488A	BTI	20011-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), F(ab')2, Rabbit (Goat) CF^TM 488A	BTI	20013	0.25 ML [2 mg/mL]	￥37,000
Anti IgG(H+L), F(ab')2, Rabbit (Goat) CF^TM 488A	BTI	20013-1	50 UL [2 mg/mL]	￥24,000
Anti IgG1 (γ1), Mouse (Goat) CF^TM 488A	BTI	20246	0.25 ML [2 mg/mL]	￥61,000
Anti IgG1 (γ1), Mouse (Goat) CF^TM 488A	BTI	20246-1	50 UL [2 mg/mL]	￥26,000
Anti IgG2a (γ2a), Mouse (Goat) CF^TM 488A	BTI	20256	0.25 ML [2 mg/mL]	￥61,000
Anti IgG2a (γ2a), Mouse (Goat) CF^TM 488A	BTI	20256-1	50 UL [2 mg/mL]	￥26,000
Anti IgG2b (γ2b), Mouse (Goat) CF^TM 488A	BTI	20266	0.25 ML [2 mg/mL]	￥61,000
Anti IgG2b (γ2b), Mouse (Goat) CF^TM 488A	BTI	20266-1	50 UL [2 mg/mL]	￥26,000
Anti IgG(H+L), Goat (Bovine) CF^TM 488A	BTI	20293	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Goat (Bovine) CF^TM 488A	BTI	20293-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Goat (Bovine) CF^TM 488A	BTI	20293-1MG	1 MG	￥58,000
Anti IgY(IgG)(H+L), Chicken (Donkey) CF^TM 488A	BTI	20166	0.5 ML [2 mg/mL]	￥57,000
Anti IgY(IgG)(H+L), Chicken (Donkey) CF^TM 488A	BTI	20166-1	50 UL [2 mg/mL]	￥24,000
Anti IgY(IgG)(H+L), Chicken (Donkey) CF^TM 488A	BTI	20166-1MG	1 MG	￥58,000
Anti IgG(H+L), Goat (Donkey) CF^TM 488A	BTI	20016	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Goat (Donkey) CF^TM 488A	BTI	20016-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Goat (Donkey) CF^TM 488A	BTI	20016-1MG	1 MG	￥58,000
Anti IgG(H+L), Guinea Pig (Donkey) CF^TM 488A	BTI	20169	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Guinea Pig (Donkey) CF^TM 488A	BTI	20169-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Guinea Pig (Donkey) CF^TM 488A	BTI	20169-1MG	1 MG	￥58,000
Anti IgG(H+L), Human (Donkey) CF^TM 488A	BTI	20074	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Human (Donkey) CF^TM 488A	BTI	20074-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Human (Donkey) CF^TM 488A	BTI	20074-1MG	1 MG	￥58,000
Anti IgG(H+L), Mouse (Donkey) CF^TM 488A	BTI	20014	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Mouse (Donkey) CF^TM 488A	BTI	20014-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Mouse (Donkey) CF^TM 488A	BTI	20014-1MG	1 MG	￥58,000
Anti IgG(H+L), Rabbit (Donkey) CF^TM 488A	BTI	20015	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Rabbit (Donkey) CF^TM 488A	BTI	20015-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Rabbit (Donkey) CF^TM 488A	BTI	20015-1MG	1 MG	￥58,000
Anti IgG(H+L), Rat (Donkey) CF^TM 488A	BTI	20027	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Rat (Donkey) CF^TM 488A	BTI	20027-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Rat (Donkey) CF^TM 488A	BTI	20027-1MG	1 MG	￥58,000
Anti IgG(H+L), Sheep (Donkey) CF^TM 488A	BTI	20024	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Sheep (Donkey) CF^TM 488A	BTI	20024-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Sheep (Donkey) CF^TM 488A	BTI	20024-1MG	1 MG	￥58,000
Anti IgY(IgG)(H+L), Chicken (Goat) CF^TM 488A	BTI	20020	0.5 ML [2 mg/mL]	￥57,000
Anti IgY(IgG)(H+L), Chicken (Goat) CF^TM 488A	BTI	20020-1	50 UL [2 mg/mL]	￥24,000
Anti IgY(IgG)(H+L), Chicken (Goat) CF^TM 488A	BTI	20020-1MG	1 MG	￥58,000
Anti IgG(H+L), Human (Goat) CF^TM 488A	BTI	20022	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Human (Goat) CF^TM 488A	BTI	20022-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Human (Goat) CF^TM 488A	BTI	20022-1MG	1 MG	￥58,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20018	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20018-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20018-1MG	1 MG	￥58,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20302	0.5 ML [2 mg/mL]	￥74,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20302-1	50 UL [2 mg/mL]	￥31,000
Anti IgG(H+L), Mouse (Goat) CF^TM 488A	BTI	20302-1MG	1 MG	￥76,000
Anti IgG(H+L), Rabbit (Goat) CF^TM 488A	BTI	20019	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Rabbit (Goat) CF^TM 488A	BTI	20019-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Rabbit (Goat) CF^TM 488A	BTI	20019-1MG	1 MG	￥58,000
Anti IgG(H+L), Rat (Goat) CF^TM 488A	BTI	20023	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Rat (Goat) CF^TM 488A	BTI	20023-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Rat (Goat) CF^TM 488A	BTI	20023-1MG	1 MG	￥58,000
Anti IgG(H+L), Human (Rabbit) CF^TM 488A	BTI	20071	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Human (Rabbit) CF^TM 488A	BTI	20071-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Human (Rabbit) CF^TM 488A	BTI	20071-1MG	1 MG	￥58,000
Anti IgG(H+L), Mouse (Rabbit) CF^TM 488A	BTI	20026	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Mouse (Rabbit) CF^TM 488A	BTI	20026-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Mouse (Rabbit) CF^TM 488A	BTI	20026-1MG	1 MG	￥58,000
Anti IgG(H+L), Rat (Rabbit) CF^TM 488A	BTI	20025	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Rat (Rabbit) CF^TM 488A	BTI	20025-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Rat (Rabbit) CF^TM 488A	BTI	20025-1MG	1 MG	￥58,000
Anti IgG(H+L), Sheep (Rabbit) CF^TM 488A	BTI	20172	0.5 ML [2 mg/mL]	￥57,000
Anti IgG(H+L), Sheep (Rabbit) CF^TM 488A	BTI	20172-1	50 UL [2 mg/mL]	￥24,000
Anti IgG(H+L), Sheep (Rabbit) CF^TM 488A	BTI	20172-1MG	1 MG	￥58,000
Anti IgM(mu chain), Human (Goat) CF^TM 488A	BTI	20347-50UL	50 UL [2 mg/mL]	￥24,000
Anti IgM(mu chain), Human (Goat) CF^TM 488A	BTI	20347-250UL	250 UL [2 mg/mL]	￥42,000
Anti IgA (α chain), Human (Goat) CF^TM 488A	BTI	20428-50UL	50 UL [2 mg/mL]	￥19,000
Anti IgA (α chain), Human (Goat) CF^TM 488A	BTI	20428-500UL	0.5 ML [2 mg/mL]	￥47,000
Anti IgA (α chain), Human (Goat) CF^TM 488A	BTI	20428-1MG	1 MG	￥49,000
Anti IgG (H&L), Fc γ specific, highly cross adsorbed, Human (Goat) CF^TM 488A	BTI	20444-50UL	50 UL [2 mg/mL]	￥24,000
Anti IgG (H&L), Fc γ specific, highly cross adsorbed, Human (Goat) CF^TM 488A	BTI	20444-500UL	0.5 ML [2 mg/mL]	￥58,000
Anti IgG (H&L), Fc γ specific, highly cross adsorbed, Human (Goat) CF^TM 488A	BTI	20444-1MG	1 MG	￥60,000
Anti IgG (H&L), Rabbit (Llama) CF^TM 488A	BTI	20449-50UL	50 UL [2 mg/mL]	￥20,000
Anti IgG (H&L), Rabbit (Llama) CF^TM 488A	BTI	20449-500UL	0.5 ML [2 mg/mL]	￥50,000
Anti IgG (H&L), Rabbit (Llama) CF^TM 488A	BTI	20449-1MG	1 MG	￥52,000
Anti IgG (H&L), Mouse (Llama) CF^TM 488A	BTI	20454-50UL	50 UL [2 mg/mL]	￥20,000
Anti IgG (H&L), Mouse (Llama) CF^TM 488A	BTI	20454-500UL	0.5 ML [2 mg/mL]	￥50,000
Anti IgG (H&L), Mouse (Llama) CF^TM 488A	BTI	20454-1MG	1 MG	￥52,000

一次抗体

品名	メーカー	品番	包装	希望販売価格
Anti GFP, Aequorea victoria (Mouse) CF^TM 488A, 9F9.F9	BTI	20215	0.1 ML [2 mg/mL]	￥69,000
Anti Biotin, (Mouse) CF^TM 488A, 3D6.6	BTI	20204	0.25 ML [2 mg/mL]	￥57,000
Anti Biotin, (Mouse) CF^TM 488A, 3D6.6	BTI	20204-1	50 UL [2 mg/mL]	￥26,000
Anti Fluorescein (FITC), (Mouse) CF^TM 488A, 1F8.1E4	BTI	20210	0.25 ML [2 mg/mL]	￥61,000
Anti Fluorescein (FITC), (Mouse) CF^TM 488A, 1F8.1E4	BTI	20210-1	50 UL [2 mg/mL]	￥30,000
Anti HA (Hemagglutinin) (Rabbit) CF^TM 488A	BTI	20238	50 UL [1 mg/mL]	￥89,000
Anti 6X His, (Mouse) CF^TM 488A, 33D10.D2.G8	BTI	20228	50 UL [1 mg/mL]	￥89,000
Anti RFP (Rabbit) CF^TM 488A	BTI	20421	100 UL [1 mg/mL]	￥69,000
Anti GST (Goat) CF^TM 488A	BTI	20424	100 UL [1 mg/mL]	￥69,000
Anti FLAG (Rabbit) CF^TM 488A	BTI	20432	100 UL [1 mg/mL]	￥72,000
Anti MYC, Human (Rabbit) CF^TM 488A	BTI	20436	100 UL [1 mg/mL]	￥72,000
Anti V5 (Rabbit) CF^TM 488A	BTI	20440	100 UL [1 mg/mL]	￥72,000

各種アッセイキット

品名	メーカー	品番	包装	希望販売価格
CF^(R)488A and 7-AAD Apoptosis Assay Kit, CF^TM 488A	BTI	30060	1 KIT [100 assays]	￥61,000
CF^(R)488A and PI Apoptosis Assay Kit, CF^TM 488A	BTI	30061	1 KIT [100 assays]	￥61,000
CF^(R)488A TUNEL Assay Apoptosis Detection Kit, CF^TM 488A	BTI	30063	1 KIT [50 rxn]	￥131,000