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記事ID : 36031
研究用

熱ショックタンパク質 70 を高感度に測定可能HSP70 ELISA キット

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HSP70 ELISA キットは、熱ショックタンパク質 70 を比色法により定量的に測定できる ELISA キットです。

背景

HSP70(Heat shock protein 70) とは

熱ショックタンパク質の Hsp 70ファミリーは 66-78 kDa 範囲のマルチプルホモログを持ち、バクテリアの DnaK に対する真核生物の等価物です。最も研究されている Hsp70 メンバーは、細胞質ストレス誘導型 Hsp70 (Hsp72)、恒常的細胞質型 Hsc70 (Hsp73)、ER局在型 BiP (Grp78) です。Hsp70 ファミリーメンバーは高度に保存された N 末端 ATPase および C 末端タンパク質結合ドメインを持っています。Hsp70 へのペプチド結合は Hsp40 にアシストされており、Hsp70 固有の ATPase 活性を刺激し、ATP 加水分解を促進させ、またペプチド結合を増進させます。Hsp70 ヌクレオチド交換や基質結合は新規合成タンパク質のフォールディング、ミスフォールドもしくは変性タンパク質のリフォールディングを調整することが知られています。

特長

  • 高感度測定
  • 特異性が高く、他のHSPとの交差性はほとんどなし
  • 定量的な測定が可能

仕様

品名 交差性 サンプルタイプ 感度 測定範囲 品番
HSP70 ELISA kit Human、Mouse、Rat 細胞ライセート、組織抽出液 200 pg/ml 780 - 50,000 pg/ml ADI-EKS-700B
HSP70 high sensitivity ELISA kit 血漿、血清、細胞ライセート、尿 90 pg/ml 0.20 - 12.5 ng/ml ADI-EKS-715
HSP70B' ELISA kit Human 細胞ライセート、血清、組織抽出液 62 pg/ml 0.156 - 10 ng/ml ADI-EKS-725A

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HSP70 ELISA キット

品名 メーカー 品番 包装 希望販売価格
HSP70 ELISA kit詳細データ ENZ ADI-EKS-700B 96 WELL
¥108,000
HSP70 High Sensitivity ELISA kit詳細データ ENZ ADI-EKS-715 96 WELL
¥109,000
HSP70B' ELISA kit詳細データ ENZ ADI-EKS-725A 1*96 WELL
¥140,000

【関連商品】

製品使用文献

■ HSP70 ELISA キット(品番:ADI-EKS-700B)

  1. Diagnostic Role for Antibodies against Heat Sock Protein 70 In Perilymphatic Fluid of Children with Cochlear Implant Surgery: A Case Control Study in Tehran, Iran: M.R. Shokrollahi, et al.; Int. J. Curr. Res. Aca. Rev. 4, 184 (2016), Application(s): HSP70 levels measured in blood samples
  2. Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping: N. Frara, et al.; BMC Musculoskelet. Dis. 17, 34 (2016), Application(s): HSP72 measured in tissue lysates
  3. Myokines In Response To A Tournament Season Among Young Tennis Players: K. Witek, et al.; Biomed Res. Int. 2016, Article ID 1460892 (2016), Application(s): Blood sampling and cytokine analysis, serum HSP analysis
  4. Physiological, Morphological and Behavioural Responses of Self-Feeding Precocial Chicks Copying with Contrasting Levels of Water Salinity during Development: A.R. Rocha, et al.; PLoS One 11, e0165364 (2016), Application(s): Hsp70 concentration fromthe erythrocyte lysates
  5. Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment: L. Shervington, et al.; J. Cancer 6, 786 (2015), Application(s): ELISA using human cell lysate
  6. Increased plasma levels of heat shock protein 70 associated with subsequent clinical conversion to mild cognitive impairment in cognitively healthy elderly: S.J. Son, et al.; PLoS One 10, e0119180 (2015), Application(s): ELISA using human plasma
  7. Lazaroids U83836E and U74389G are potent, time-dependent inhibitors of caspase-1: M. Kawarski, et al.; Chem. Biol. Drug Des. 86, 1049 (2015), Application(s): Analysis of tissue lysates
  8. Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): EIA using mouse tissue extract
  9. Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R844 (2007), Application(s): EIA using human vastus lateralis muscle tissue

■ HSP70 高感度 ELISA キット(品番:ADI-EKS-715)

  1. The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats: D. Stygar, et al.; Cell Stress Chaperones 24, 427 (2019), Application(s): HSP70 levels in rat liver tissue
  2. Heat shock protein 70 and anti-heat shock protein 70 antibodies in patients with chronic glomerulonephritis: N. Chebotareva, et al.; Cell Stress Chaperones 23, 1229 (2018), Application(s): HSP70 levels in human urine
  3. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis : M. Sevin, et al.; Nat. Commun. 9, 1431 (2018), Application(s): HSP90a levels in human serum
  4. Resistance training and L-arginine supplementation are determinant in genomic stability, cardiac contractility and muscle mass development in rats: G.P. Stefani, et al.; PLoS One 13, e0204858 (2018)
  5. Treatment with Geranylgeranylacetone Induces Heat Shock Protein 70 and Attenuates Neonatal Hyperoxic Lung Injury in a Model of Bronchopulmonary Dysplasia: S. Tokuriki, et al.; Lung 195, 469 (2017), Application(s): HSP70 ELISA on Human Neonatal Serum
  6. Evaluation of tympanic temperature and thermal sensation responses during exercise to verify the positive effects of wearing germanium-coated functional clothing: S.Y. Cho, et al.; J. Phys. Ther. Sci. 28, 1860 (2016), Application(s): ELISA, human blood and serum levels
  7. Evaluation of tympanic temperature and thermal sensation responses during exercise to verify the positive effects of wearing germanium-coated functional clothing: S.Y. Cho, et al.; J. Phys. Ther. Sci. 28, 1860 (2016), Application(s): ELISA, human blood and serum levels
  8. Salivary extracellular heat shock protein 70 (eHSP70) levels increase after 59 min of intense exercise and correlate with resting salivary secretory immunoglobulin A (SIgA) levels at rest: Y. Murase, et al.; Cell Stress Chaperones 21, 261 (2016)
  9. Urinary Biomarkers Indicative of Apoptosis and Acute Kidney Injury in the Critically Ill: S.T. Vaara, et al.; PLoS One 11, e0149956 (2016), Application(s): Determination of plasma and urine HSP70
  10. A comparison of two commercially available ELISA methods for the quantification of human plasma heat shock protein 70 during rest and exercise stress: B.J. Lee, et al.; Cell Stress Chaperones 20, 917 (2015), Application(s): ELISA using human plasma
  11. Analysis of serum heat shock protein 70 (HSPA1A) concentrations for diagnosis and disease activity monitoring in patients with rheumatoid arthritis: S.R. Najafizageh, et al.; Cell Stress Chaperones 20, 537 (2015), Application(s): Immunosorbent assay
  12. COPD and levels of Hsp70 (HSPA1A) and Hsp27 (HSPB1) in plasma and lymphocytes among coal workers: a case-control study: X. Cui, et al.; Cell Stress Chaperones 20, 473 (2015), Application(s): EIA using human plasma
  13. Early inflammatory response in polytraumatized patients: Cytokines and heat shock proteins. A pilot study: M.C. Guisasola, et al.; Orthop. Traumatol. Surg. Res. 101, 607 (2015), Application(s): ELISA using human serum
  14. Human monocyte heat shock protein 72 responses to acute hypoxic exercise after 3 days of exercise heat acclimation: B.J. Lee, et al.; Biomed. Res. Int. 2015, 849809 (2015), Application(s): ELISA using human plasma
  15. Hsp72 Is a Novel Biomarker to Predict Acute Kidney Injury in Critically Ill Patients: L.E. Morales-Buenrostro, et al.; PLoS One 9, e109407 (2014)
  16. Induction of Heat Shock Protein 70 Ameliorates Ultraviolet-Induced Photokeratitis in Mice: A. Lennikov, et al.; Int. J. Mol. Sci. 14, 2175 (2013), Application(s): EIA using mouse cornea cell extracts
  17. Response of circulating heat shock protein 70 and anti-heat shock protein 70 antibodies to catheter ablation of atrial fibrillation: J. Kornej, et al.; J. Transl. Med. 11, 49 (2013), Application(s): EIA using human plasma samples
  18. Electrophysiological correlation and prognostic impact of heat shock protein 27 in atrial fibrillation: Y.F. Hu, et al.; Circ. Arrhythm. Electrophysiol. 5, 334 (2012), Application(s): ELISA using human serum
  19. Extracellular hsp70 Release in Canine Steroid Responsive Meningitis-Arteritis: S. A. Moore, et al.; Vet. Immunol. Immunopathol. 145, 129 (2012), Application(s): ELISA using canine serum and CSF samples
  20. Plasma heat shock protein 72 as a biomarker of sarcopenia in elderly people: K. Ogawa, et al.; Cell Stress Chaperones 17, 349 (2012), Application(s): EIA using human plasma samples
  21. Recovery from ischemic acute kidney injury by spironolactone administration: K. S?nchez-Pozos, et al.; Nephrol. Dial. Transplant. 27, 3160 (2012), Application(s): Urinary Hsp72 protein levels
  22. Distinguishing integral and receptor-bound heat shock protein 70 (Hsp70) on the cell surface by Hsp70-specific antibodies: G. Multhoff & L. Hightower; Cell Stress Chaperones 16, 251 (2011), Application(s): EIA of extracellular HSP in serum and plasma samples
  23. Plasma levels of Hsp70 and anti-Hsp70 antibody predict risk of acute coronary syndrome: X. Zhang, et al.; Cell Stress Chaperones 15, 675 (2010), Application(s): EIA using human plasma

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