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フローサイトメトリー解析やイメージングに有用 PROTEOSTAT® アグリソーム検出キット

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メーカー名：Enzo Life Sciences,Inc.

PROTEOSTAT® アグリソーム検出キットは、アグリソームやアグリソーム様凝集体をフローサイトメトリーやイメージングで解析します。

FAQ あります！

使用目的

PROTEOSTAT® アグリソーム検出キットには、488 nm で励起する赤色蛍光分子ローター色素が含まれており、固定細胞や透過処理済み細胞のアグリソームやアグリソーム様封入体内に存在する、変性カーゴタンパク質を特異的に検出します。検出試薬はアグリソーム形成中に生産された小胞内の凝集タンパク質と結合することで強く蛍光を発します。そしてオートファジー、プロテアソーム経路の様々な条件下でバリデートされています。

キットには、プロテアソーム阻害剤である MG-132 がポジティブコントロールとして、また核の対比染色試薬も入っています。

  • 文献で使用されているサンプル一覧はこちら PDF

特長

  • セルベースアッセイによる薬剤応答アッセイ：実際の生細胞条件下での神経変性疾患に関与するインヒビターを同定可能
  • 信頼性が高く簡便：非生理的なタンパク質のミューテーションや遺伝子組換え細胞株不要
  • 固定化細胞アッセイ：凝集したタンパク質とアグリソーム形成に関わる様々なタンパク質間の相互作用を解析可能
  • フローサイトメトリーでアグリソーム蓄積を簡単定量

構成内容

  • PROTEOSTAT® アグリソーム検出試薬
  • Hoechst 33342 Nuclear stain
  • プロテアソームインヒビター（MG-132）
  • アッセイバッファー（×10）

テックノート

使用例

PROTEOSTAT アグリソーム検出キットの使用例

Treatment Mean（FL3）Signal
Control 113
Treatment（5μM MG-132） 335


図1　フローサイトメトリーによるアグリソーム解析
Jurkat細胞を5μM MG-132で一晩37℃誘導したものとmockを用意。処理後、細胞を固定し PROTEOSTAT® 検出試薬とインキュベートした。フローサイトメトリーで検出したヒストグラムを示す。
MG-132処理細胞では、赤色蛍光シグナルが約3倍増加した。

PROTEOSTAT 検出試薬で検出されるアグリソームはFITC標識ユビキチン抗体と共通の局在
図2　PROTEOSTAT® 検出試薬で検出されるアグリソームはFITC標識ユビキチン抗体と共通の局在を示す。

α-syn混合物はM17神経芽細胞系に内在する細胞原形質膜で凝集体を形成する。

図3　α-syn 混合物は M17 神経芽細胞系に内在する細胞原形質膜で凝集体を形成する。
α-syn モノマーで処理した M17 細胞（M）、ソニケートした α-syn PFFs で処理した M17 細胞（F）、α-syn 混合物で処理した M17 細胞（M+F）。4日後、M17 細胞を固定前に3回洗浄し、それから PROTEOSTAT® アグリソーム検出試薬（緑）で染色、α-syn（赤）および β-catenin（グレイ）に対する抗体を使い、免疫染色を行った。スケールバー = 20 µm。
(“Fibril growth and seeding capacity play key roles in α-synuclein-mediated apoptotic cell death. A-L Mahul-Mellier, et al.; Cell Death & Differentiation (2015).” (doi:10.1038/cdd.2015.79))

オートファジーに関連するERストレスによって誘導されたタンパク質アグリソームのフローサイトメトリー解析

図4　オートファジーに関連するERストレスによって誘導されたタンパク質アグリソームのフローサイトメトリー解析
K562 細胞を CQ（25, 50, 75 µM）、thapsigargin（Tg ：100 nM）、MG-132（5 µM）で24時間処理、もしくは未処理を用意した。その後、固定・透過処理を行い、PROTEOSTAT® アグリソーム検出試薬（1:10,000 希釈）を室温で30分反応させた。その後、細胞（30,000）を BD LSR II、610/20nm（青）チャンネルで解析した。
(Courtesy of the Flow Cytometry Core Facility, Blizard Institute, Queen Mary University of London, London, UK.)

オートファジーに関連するERストレスによって誘導されたアグリソームやアグリソーム様封入体 (ALSI) の落射蛍光顕微鏡画像

図5　オートファジーに関連するERストレスによって誘導されたアグリソームやアグリソーム様封入体（ALSI）の落射蛍光顕微鏡画像
A) 未処理の K562 細胞、B) MG-132（5 µM）、C) CQ（50 µM）、D) thapsigargin（Tg： 100 nM）で24時間処理、その後、固定・透過処理を行い、PROTEOSTAT® アグリソーム検出試薬（1:10,000 希釈）を室温で30分反応させた。PROTEOSTAT® アグリソーム染色には、アグリソーム、ALIS 細胞を赤色で、DAPI 染色を青色で示す。
(Courtesy of the Flow Cytometry Core Facility, Blizard Institute, Queen Mary University of London, London, UK.)

ENZ-51035_fig6.gif

図6.　ERストレスおよび不完全オートファジー誘導試薬を処理したK562細胞のAPF解析
K562細胞を、ERストレスおよび不完全オートファジーを誘導する試薬Thapsigargin（Tg、0.1µM）、25、50および75µMのChloroquine（CQ）をそれぞれ24時間処理した。プロテアソーム阻害剤 MG-132 (5 µM) をポジティブコントロールとして24 時間使用した。ペレット化した細胞を固定し、透過処理した。次に細胞を、メーカーの指示に従って300µl PROTEOSTAT® Aggresome Detection reagent (Enzo Life Sciences)および1µg/ml DAPI (細胞周期決定用)を用いて室温で30分間標識した。 G1期を上回るS期のアグレソーム傾向因子（APF）およびS期を上回るG2m期のアグリソーム傾向因子（APF）の相対的増加を各治療について決定し、対照値と比較した。 ER ストレス誘導物質である Tg と 50 µM CQ は両方とも、対照と比較して G1期よりも S期でアグレソームをより上方制御することが示された。一方、プロテアソーム阻害剤である MG-132 および 50 µM CQ は、コントロールや S期よりも G2m 期でアグリソームを下方制御することが示された。(Courtesy of the Flow Cytometry Core Facility, Blizard Institute, Queen Mary University of London, London, UK.)

ENZ-51035_fig7.gif

図7. フローサイトメトリーによるアグリソーム解析
Jurkat 細胞を 0.2% DMSO で模擬誘導または、5 µM MG-132 で 37℃ で一晩誘導した。処理後、細胞を固定し、Fluorescein-p62 Ab (希釈率 1:500) でインキュベートし、洗浄後、フローサイトメトリーで分析した。(FL1 チャネル:488 nm レーザー)
MG-132 処理細胞では、Fluorescein-p62 Ab シグナルが約 2.5 倍増加した。

ENZ-51035_fig8.gif

図8. HeLa 細胞におけるアグリソームとTubulinの解析
細胞を5µM MG-132 で 12 時間処理し、ProteoStat® アグリソーム色素で検出した(左上、緑色で表示) 。 また、Alexa Fluor® 647-Tubulin 抗体で染色した(左下、赤色で表示)。 合成画像（右）において、Tubulinとの共局在を示した。(蛍光顕微鏡使用)

ENZ-51035_fig9.gif

図9. HeLa 細胞におけるアグリソームとp62の解析
細胞を5µM MG-132 で 12 時間処理し、ProteoStat® アグリソーム色素で検出した(左上、赤色で表示) 。 また、フルオレセイン p62 抗体で染色した(右上、緑色で表示)。 合成画像（中央下）において、p62 との共局在を示した。(蛍光顕微鏡使用)

ENZ-51035_fig10.gif

図10. HeLa 細胞におけるアグリソーム解析およびHoechst 33342による対比染色
細胞を5µM MG-132 で 12 時間処理し、ProteoStat® アグリソーム色素で検出した(赤色) 。 また、Hoechst 33342 で対比染色した(青色)。左のコントロール パネルはMG-132未処理。(蛍光顕微鏡使用)

PROTEOSTAT® アグリソーム検出キット

品名 メーカー 品番 包装 希望販売価格
PROTEOSTAT(R) Aggresome detection kit詳細データ ENZ ENZ-51035-K100 1 KIT
[100 flow cytometry assays or 200 microscopy assays] 		￥86,000
PROTEOSTAT(R) Aggresome detection kit詳細データ ENZ ENZ-51035-0025 1 KIT
[25 flow cytometry assays or 50 microscopy assays] 		￥30,000

製品使用文献

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  76. Cigarette smoke induced autophagy-impairment accelerates lung aging, COPD-emphysema exacerbations and pathogenesis: N. Vij, et al.; Am. J. Physiol. Cell. Physiol. 314, C73 (2018), Abstract;
  77. Culling less fit neurons protects against amyloid-β-induced brain damage and cognitive and motor decline: D.S. Coelho, et al.; Cell Rep. 25, 3661 (2018), Application(s): Confocal microscopy using fly brain tissue section, Abstract; Full Text
  78. Ethanol induced disordering of pancreatic acinar cell endoplasmic reticulum: an ER stress/defective unfolded protein response model: R.T. Waldron, et al.; Cell. Mol. Gastroenterol. Hepatol. 5, 479 (2018), Application(s): Confocal microscopy using AR42J cells, Abstract; Full Text
  79. iPSCs from a hibernator provide a platform for studying cold adaptation and its potential medical applications: J. Ou, et al.; Cell 173, 851 (2018), Application(s): Fluorescence microscopy using squirrel neurons, Abstract; Full Text
  80. Novel polyubiquitin imaging system, PolyUb-FC, reveals that K33-linked polyubiquitin is recruited by SQSTM1/p62: Y. Nibe, et al.; Autophagy 14, 347 (2018), Application(s): Fluorescence microscopy using HEK293T cells, Abstract; Full Text
  81. Spermine increases acetylation of tubulins and facilitates autophagic degradation of prion aggregates: K. Phadwal, et al.; Sci. Rep. 8, 10004 (2018), Application(s): Confocal microscopy with CAD and SMB cells, Abstract; Full Text
  82. The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients: S. Koyuncu, et al.; Nat. Commun. 9, 2886 (2018), Application(s): Fluorescence microscopy using pluripotent stem cells, Abstract; Full Text
  83. A leptospiral AAA+ chaperone-Ntn peptidase complex, HslUV, contributes to the intracellular survival of Leptospira interrogans in hosts and the transmission of leptospirosis: S.L. Dong, et al.; Emerg. Microbes Infect. 6, e105 (2017), Application(s): Protein aggresome detection in intracellular leptospires, Abstract; Full Text
  84. Augmentation of S-Nitrosoglutathione Controls Cigarette Smoke-Induced Inflammatory-Oxidative Stress and Chronic Obstructive Pulmonary Disease-Emphysema Pathogenesis by Restoring Cystic Fibrosis Transmembrane Conductance Regulator Function: M. Bodas, et al.; Antioxid. Redox Signal. 27, 433 (2017), Application(s): Human and murine lung tissue, Abstract; Full Text
  85. Characterization of cadmium chloride-induced BiP accumulation in Xenopus laevis A6 kidney epithelial cells: C.S. Shirriff, et al.; Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 191, 117 (2017), Application(s): Immunocytochemical analysis and laser scanning confocal microscopy, Abstract;
  86. Endoplasmic Reticulum Stress Response in Arabidopsis Roots: Y. Cho, et al.; Front. Plant Sci. 8, 144 (2017), Application(s): Fluorescence Imaging of Arabidopsis roots, Abstract; Full Text
  87. FKBP8 protects the heart from hemodynamic stress by preventing the accumulation of misfolded proteins and endoplasmic reticulum-associated apoptosis in mice: T. Misaka, et al.; J. Mol. Cell. Cardiol. 114, 93 (2017), Abstract;
  88. Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice: D. Zhou, et al.; Sci. Rep. 7, 8131 (2017), Application(s): Mouse Oocyte Samples, Abstract; Full Text
  89. OSM mitigates post-infarction cardiac remodeling and dysfunction by up-regulating autophagy through Mst1 suppression: J. Hu, et al.; Biochim. Biophys. Acta 1863, 1951 (2017), Abstract;
  90. p53 amyloid formation leading to its loss of function: implications in cancer pathogenesis: S. Ghosh, et al.; Cell Death Differ. 24, 1784 (2017), Application(s): p53 aggregate formation in SY5Y cells, Abstract; Full Text
  91. Polyethylene glycol-functionalized poly (Lactic Acid-co-Glycolic Acid) and graphene oxide nanoparticles induce pro-inflammatory and apoptotic responses in Candida albicans-infected vaginal epithelial cells: R.D. Wagner, et al.; PLoS One 12, e0175250 (2017), Abstract; Full Text
  92. Subcellular localization of the five members of the human steroid 5α-reductase family: A. Scaglione, et al.; Biochim. Open 4, 99 (2017), Application(s): Fluorescence Microscopy of HeLa cells, Abstract; Full Text
  93. Targeting mitochondrial dysfunction can restore antiviral activity of exhausted HBV-specific CD8 T cells in chronic hepatitis B: P. Fisicaro, et al.; Nat. Med. 23, 327 (2017), Abstract;
  94. A fast and specific method to screen for intracellular amyloid inhibitors using bacterial model systems: S. Navarro, et al.; Eur. J. Med. Chem. 121, 785 (2016), Application(s): Confocal microscopy, Abstract;
  95. Ad-HGF improves the cardiac remodeling of rat following myocardial infarction by upregulating autophagy and necroptosis and inhibiting apoptosis: J. Liu, et al.; Am. J. Transl. Res. 8, 4605 (2016), Abstract; Full Text
  96. Airway exposure to e-cigarette vapors impairs autophagy and induces aggresome formation: P.C. Shivalingappa, et al.; Antioxid. Redox Signal. 24, 186 (2016), Abstract;
  97. Bile acids protect expanding hematopoietic stem cells from unfolded protein stress in fetal liver: Y. Sigurdsson, et al.; Cell Stem Cell 18, 522 (2016), Abstract;
  98. Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation: C. Mukhopadhyay, et al.; PNAS 113, E8228 (2016), Abstract; Full Text
  99. Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS: A. Valdes, et al.; J. Proteome Res. 15, 1971 (2016), Abstract;
  100. Distinct roles for intracellular and extracellular lipids in hepatitis C virus infection: S. Narayanan, et al.; PLoS One 11, e0156996 (2016), Application(s): Multiphoton microscopy using human hepatocellular carcinoma HuH7.5.1 cells, Abstract; Full Text
  101. Lin28a protects against postinfarction myocardial remodeling and dysfunction through Sirt1 activation and autophagy enhancement: Y. Hao, et al.; Biochem. Biophys. Res. Commun. 479, 833 (2016), Application(s): Aggresome and p62 detection, Abstract;
  102. LRRK2 interferes with aggresome formation for autophagic clearance: Y. Bang, et al.; Mol. Cell. Neurosci. 75, 71 (2016), Application(s): Protein aggregate analysis, Abstract;
  103. Luteolin alleviates post-infarction cardiac dysfunction by up-regulating autophagy through Mst1 inhibition: J. Hu, et al.; J. Cell. Mol. Med. 20, 147 (2016), Application(s): Confocal microscopy using mouse cardiomyocytes, Abstract; Full Text
  104. Monitoring of dipeptidyl peptidase-IV (DPP-IV) activity in patients with mucopolysaccharidoses types I and II on enzyme replacement therapy - Results of a pilot study: K. Hetmanczyk, et al.; Clin. Biochem. 49, 458 (2016), Application(s): Plasma DPP-IV enzyme assay, Abstract;
  105. A fast and specific method to screen for intracellular amyloid inhibitors using bacterial model systems: S. Navarro, et al.; Eur. J. Med. Chem. (2015), Application(s): Confocal microscopy, Abstract;
  106. Amyloidogenic lysozymes accumulate in the endoplasmic reticulum accompanied by the augmentation of ER stress signals: Y. Kamada, et al.; Biochim. Biophys. Acta 1850, 1107 (2015), Application(s): Microscopy, Abstract;
  107. Conophylline protects cells in cellular models of neurodegenerative diseases by inducing mammalian target of rapamycin (mTOR)-independent autophagy: Y. Sasazawa, et al.; J. Biol. Chem. 290, 6168 (2015), Abstract;
  108. Decreased proteasomal function accelerates cigarette smoke-induced pulmonary emphysema in mice: Y. Yamada, et al.; Lab. Invest. 95, 625 (2015), Application(s): Aggresome detection by fluorescence microscopy in fibroblasts, Abstract;
  109. Defective autophagy is a key feature of cerebral cavernous malformations: S. Marchi, et al.; EMBO Mol. Med. 7, 1403 (2015), Application(s): Aggresome detection in aggregated proteins and aggresome‐like inclusion bodies in fixed and permeabilized samples, Abstract; Full Text
  110. Fibril growth and seeding capacity play key roles in α-synuclein-mediated apoptotic cell death: A.L. Mahul-Mellier, et al.; Cell Death Differ. 22, 2107 (2015), Abstract;
  111. In vitro administration of gold nanoparticles functionalized with MUC-1 protein fragment generates anticancer vaccine response via macrophage activation and polarization mechanism: T. Mocan, et al.; J. Cancer 6, 583 (2015), Application(s): Aggresome detection by fluorescence microscopy in peritoneal macrophages, Abstract; Full Text
  112. Intensified autophagy compromises the efficacy of radiotherapy against prostate cancer: M.I. Koukourakis, et al.; Biochem. Biophys. Res. Commun. 461, 268 (2015), Application(s): Fluorescence microscopy , Abstract;
  113. Mevalonate pathway regulates cell size homeostasis and proteostasis through autophagy: T.P. Miettinen, et al.; Cell Rep. 13, 2610 (2015), Application(s): Flow cytometry analysis of protein aggregation using Jurkat, U2OS, Kc167 and HUVEC cells, Abstract;
  114. MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomib: S. Jagannathan, et al.; Leukemia 29, 727 (2015), Application(s): Detection of protein aggregates by fluorescence microscopy in multiple myeloma cell lines, Abstract; Full Text
  115. Molecular chaperone GRP78 enhances aggresome delivery to autophagosomes to promote drug resistance in multiple myeloma: M.A. Abdel Malek, et al.; Oncotarget 6, 3098 (2015), Application(s): Confocal Microscopy, Abstract; Full Text
  116. Pressure overload-induced cardiac dysfunction in aged male adiponectin knockout mice is associated with autophagy deficiency: J.W. Jahng, et al.; Endocrinology 156, 1667 (2015), Abstract;
  117. Protein kinase C-dependent growth-associated protein 43 phosphorylation regulates gephyrin aggregation at developing GABAergic synapses: C.Y. Wang, et al.; Mol. Cell. Biol. 35, 1712 (2015), Abstract;
  118. Schwann cells contribute to neurodegeneration in transthyretin amyloidosis: T. Murakami, et al.; J. Neurochem. 134, 66 (2015), Abstract;
  119. Cationic polystyrene nanospheres induce autophagic cell death through the induction of endoplasmic reticulum stress: H.W. Chiu, et al.; Nanoscale 7, 736 (2014), Abstract;
  120. Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen: S.M. Usmani, et al.; Nat. Commun. 5, 3508 (2014), Application: Amyloid detection in semen, Abstract;
  121. Distinct patterns of HSP30 and HSP70 degradation in Xenopus laevis A6 cells recovering from thermal stress: S. Khan, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 168, 1 (2014), Application(s): Detection of aggresomes in Xenopus laevis cells using fluorescence microscopy, Abstract;
  122. Dynein function and protein clearance changes in tumor cells induced by a kunitz-type molecule, amblyomin-x: M.T. Pacheco, et al.; PLoS One 9, e111907 (2014), Application(s): Detection of aggresomes by flow cytometry, Abstract; Full Text
  123. Higher vulnerability and stress sensitivity of neuronal precursor cells carrying an alpha-synuclein gene triplication: A. Flierl, et al.; PLoS One 9, e112413 (2014), Application(s): Detection of protein aggregates by fluorescence microscopy and flow cytometry in neuronal precursor cells, Abstract; Full Text
  124. Human stefin B role in cell's response to misfolded proteins and autophagy: M. Polajnar, et al.; PLoS One 9, e102500 (2014), Application(s): Detection of protein aggregates in primary astrocytes, Abstract; Full Text
  125. Novel estradiol analogue induces apoptosis and autophagy in esophageal carcinoma cells: E. Wolmarans, et al.; Cell Mol. Biol. Lett. 19, 98 (2014), Abstract;
  126. Preconditioning stimulus of proteasome inhibitor enhances aggresome formation and autophagy in differentiated SH-SY5Y cells: Y. Bang, et al.; Neurosci. Lett. 566, 263 (2014), Abstract;
  127. Protein deubiquitination during oocyte maturation influences sperm function during fertilisation, antipolyspermy defense and embryo development: Y.J. Yi, et al.; Reprod. Fertil. Dev. (2014), Application(s): Detection of protein aggregates in oocytes, Abstract;
  128. Protein expression pattern of PAWP in bull spermatozoa is associated with sperm quality and fertility following artificial insemination: C.E. Kennedy, et al.; Mol. Reprod. Dev. 81, 436 (2014), Abstract;
  129. Serine/threonine kinase 16 and MAL2 regulate constitutive secretion of soluble cargo in hepatic cells: J.G. In, et al.; Biochem. J. 463, 201 (2014), Abstract;
  130. SGTA regulates the cytosolic quality control of hydrophobic substrates: L. Wunderley, et al.; J. Cell. Sci. 127, 4728 (2014), Application(s): Dual staining with ProteoStat® dye, Abstract; Full Text
  131. The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget 5, 6252 (2014), Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay, Abstract; Full Text
  132. Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules: M.U. Cheema, et al.; Physiol. Rep. 1, e00064 (2013), Application(s): Labeling of kidney homogenates, labeled particles sorted by flow cytometry and identification by LC-MS/MS , Abstract; Full Text
  133. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death: P. Magnaghi, et al.; Nat. Chem. Biol. 9, 548 (2013), Application(s): Detection of aggresomes in human colon carcinoma HCT116 cells using fluorescence microscopy, Abstract;
  134. Environmental stresses induce misfolded protein aggregation in plant cells in a microtubule-dependent manner: Y. Nakajima, et al.; Int. J. Mol. Sci. 14, 7771 (2013), Application(s): Detection of aggresomes using fluorescence microscopy, Abstract; Full Text
  135. In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-β-estradiol analogue in breast adenocarcinoma cells: D.S. Nkandeu, et al.; Cell. Biochem. Funct. 31, 566 (2013), Abstract;
  136. Increased generation of cyclopentenone prostaglandins after brain ischemia and their role in aggregation of ubiquitinated proteins in neurons: H. Liu, et al.; Neurotox. Res. 24, 191 (2013), Abstract;
  137. Macrolide antibiotics block autophagy flux and sensitize to bortezomib via endoplasmic reticulum stress-mediated CHOP induction in myeloma cells: S. Moriya, et al.; Int. J. Oncol. 42, 1541 (2013), Application(s): Detection of aggresomes using flow cytometry, Abstract; Full Text
  138. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2: Y. Maejima, et al.; Nat. Med. 19, 1478 (2013), Application(s): Detection of aggresomes in mouse heart sections using fluorescence microscopy, Abstract; Full Text
  139. N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions: B. Jeric, et al.; Biochim. Biophys. Acta 1833, 2254 (2013), Application(s): Detection of aggresomes using fluorescence microscopy, Abstract;
  140. The ubiquitin proteasome system regulates the stability and activity of the glucose sensor glucokinase in pancreatic beta cells: A. Hofmeister-Brix, et al.; Biochem. J. 456, 173 (2013), Abstract; Full Text
  141. VCP Phosphorylation-Dependent Interaction Partners Prevent Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cells: C.C. Yu, et al.; PLoS One 8, e55724 (2013), Application(s): Aggresome detection in AGS human gastric epithelial cells, Abstract; Full Text
  142. Zerumbone, an electrophilic sesquiterpene, induces cellular proteo-stress leading to activation of ubiquitin-proteasome system and autophagy: K. Ohnishi, et al.; BBRC 430, 616 (2013), Application(s): Aggresome detection in mouse hepatocytes, Abstract;
  143. Autophagy in idiopathic pulmonary fibrosis: A.S. Patel, et al.; PLoS One 7, e41394 (2012), Application(s): Detection of aggresomes in lung tissue sections using fluorescence microscopy, Abstract; Full Text
  144. Decreased proteasomal activity causes age-related phenotypes and promotes the development of metabolic abnormalities: U. Tomaru, et al.; Am. J. Pathol. 180, 963 (2012), Abstract;
  145. Mutations in the area composita protein αT-catenin are associated with arrhythmogenic right ventricular cardiomyopathy: J. van Hengel, et al.; Eur. Heart J. 34, 201 (2012), Abstract;
  146. Quantitative analysis of α-synuclein solubility in living cells using split GFP complementation: A. Kothawala, et al.; PLoS One 7, e43505 (2012), Application(s): Aggresome detection in HeLa cells, Abstract; Full Text
  147. Multiple aggregates and aggresomes of C-terminal truncated human αA-crystallins in mammalian cells and protection by αB-crystallin: I. Raju, et al.; PLoS One 6, e19876 (2011), Application(s): Aggresome detection in HeLa cells, Abstract; Full Text
  148. Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies: D. Shen, et al.; Cell Biochem. Biophys. 60, 173 (2011), Abstract; Full Text

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