ヒトiPS 細胞/ES細胞用のフィーダーフリー培養、シングルセルクローニングが可能なゼノフリー培地 ヒトiPS 細胞/ES細胞用培地 NutriStem® hPSC XF

NutriStem® 使用文献情報

NutriStem® and Cardiomyocytes

1. P. Menasché et al. Human embryonic stem cell-derived cardiac progenitors for severe heart failure treatment: first clinical case report. European heart journal (2015): ehv189.
2. L. Jacquet et al. Three Huntington?s Disease Specifi c Mutation-Carrying Human Embryonic Stem Cell Lines Have Stable Number of CAG Repeats upon In Vitro Diff erentiation into Cardiomyocytes. PloS one 10.5, 2015
3. S. Rajasingh et al. Generation of Functional Cardiomyocytes from Efficiently Generated Human iPSCs and a Novel Method of Measuring Contractility. PloS one 10.8, 2015: e0134093.
4. W. Siqin et al. Spider silk for xeno-free long-term self-renewal and differentiation of human pluripotent stem cells. Biomaterials 35.30 (2014): 8496-8502.
5. E. Di Pasquale et al. Generation of human cardiomyocytes: a differentiation protocol from feeder-free human induced pluripotent stem cells. JoVE (Journal of Visualized Experiments) 76 (2013): e50429-e50429
6. G. Földes and M. Mioulane. High-content imaging and analysis of pluripotent stem cell derived cardiomyocytes. Imaging and Tracking Stem Cells. Humana Press, 2013.
7. P.W. Burridge and E.T Zambidis Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes. Pluripotent Stem Cells. Humana Press, 2013.

Differentiation of Pluripotent Stem Cells

1. A.P. Reyes et al. Xeno-Free and Defined Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Functionally Integrate in a Large-Eyed Preclinical Model. Stem Cell Reports, 2015
2. M.V. Krivega et al. Cyclin E1 plays a key role in balancing between totipotency and differentiation in human embryonic cells. Mol. Hum. Reprod, 2015
3. S. Rajasingh et al., Generation of Functional Cardiomyocytes from Efficiently Generated Human iPSCs and a Novel Method of Measuring Contractility. PloS one, 2015

Growing Methods of hPSC and IPSC (Derivation, Expansion, Scaling up and Suspension)

1. Y.Y. Lipsitz, P.W. Zandstra, Human pluripotent stem cell process parameter optimization in a small scale suspension bioreactor. BMC Proceedings, 2015
2. S. Eminli-Meissner et al. A novel four transfection protocol for deriving iPS cell lines from human blood-derived endothelial progenitor cells (EPCs) and adult human dermal fibroblasts using a cocktail of non-modifi ed reprogramming and immune evasion mRNAs. Sientific Poster, REPROCELL, 2015
3. S. Wu et al. Efficient passage of human pluripotent stem cells on spider silk matrices under xeno-free conditions. Cellular and Molecular Life Sciences, 2015
4. S. Gregory et al. Autophagic response to cell culture stress in pluripotent stem cells. Biochemical and Biophysical Research Communications, doi:10.1016/j.bbrc.2015.09.080, 2015
5. H Tateno et al., Undifferentiated Cell Detection Method And Complex Carbohydrate Detection Method. US Patent 20,150,204,870, 2015
6. S. Herz, Optimization of RNA-based transgene expression by targeting Protein Kinase R. Dissertation for the degree “Doctor rerum naturalium”, 2015
7. J. Lenzi et al., ALS mutant FUS proteins are recruited into stress granules in induced Pluripotent Stem Cells (iPSCs) derived motoneurons. Disease Models & Mechanisms, 2015
8. N. Desai, P Rambhia and A. Gishto, Human embryonic stem cell cultivation: historical perspective and evolution of xeno-free culture systems. Reproductive Biology and Endocrinology 13.1 (2015): 9.
9. T. Yokobori et al., Intestinal epithelial culture under an air ‐ liquid interface: a tool for studying human and mouse esophagi. Diseases of the Esophagus, 2015
10. T Cerbini et al., Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor, JoVE (Journal of Visualized Experiments), 2015
11. L Healy, L Ruban, Derivation of Induced Pluripotent Stem Cells, Atlas of Human Pluripotent Stem Cells in Culture, 2015
12. N.Y. Thakar et al., TRAF2 recruitment via T61 in CD30 drives NF κ B activation and enhances hPSC survival and proliferation, Molecular Biology of the Cell, 2015
13. M. Amit, J. Itskovitz-eldor. Novel Methods and Culture Media for Culturing Pluripotent Stem Cells. US Patent 20,130,236,961, 2013
14. M. Amit, J. Itskovitz-Eldor. Atlas of Human Pluripotent Stem Cells: Derivation and Culturing. Stem Cell Biology and Regenerative Medicine, 2012

For Clinical Applications- Derivation and Expansion of hPSC and IPSC

1. A. P. Reyes et al. Xeno-Free and Defined Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Functionally Integrate in a Large-Eyed Preclinical Model. Stem Cell Reports, Vol. 6, 2016
2. M. Di Salvio et al. Pur-alpha functionally interacts with FUS carrying ALS-associated mutations. Cell Death & Disease, 2015
3. L. de Oñate et al. Research on Skeletal Muscle Diseases Using Pluripotent Stem Cells. DOI: 10.5772/60902, 2015
4. P. Menasché et al., Towards a Clinical Use of Human Embryonic Stem Cell-Derived Cardiac Progenitors: A Translational Experience.
5. T. Seki, K. Fukuda, Methods of induced pluripotent stem cells for clinical application, World Journal of Stem Cells, 2015
6. S. Abbasalizadeh, H. Baharvand. Technologies progress and challenges towards cGMP manufacturing of human pluripotent stem cells based therapeutic products for allogeneic and autologous cell therapies. Biotechnology Advances, 2013.
7. J. Durruthy-Durruthy et al., Rapid and Effi cient Conversion of Integration-Free Human Induced Pluripotent Stem Cells to GMP-Grade Culture Conditions. PlOS one, 2014