記事ID ： 9315

精製不要・わずか30分で抗体を標識 Mix-n-Stain™ 抗体標識キット

カテゴリから探す > 抗体アッセイ > 抗体・タンパク質標識 > 蛍光色素標識

メーカー名：Biotium, Inc.

Mix-n-Stain™ 抗体標識キットは、1 - 100 ug の抗体をわずか30分で簡単に標識できるキットです。標識した抗体の回収率は 100% です。

共有結合により標識するため、標識した抗体は長期間安定して保存することができ、マルチカラーイメージングなどに最適です。

CF® Dyeの選択ガイドはこちらです。

使用目的

Mix-n-Stain™ CF® Dye 抗体標識キットを使用すると、蛍光標識抗体を簡単に調製することができます。

抗体と、キットに含まれる反応バッファー、CF® Dye を混合するだけで、市販の蛍光標識抗体に匹敵した品質の CF® Dye 標識抗体が得られます。分離の工程は不要です。CF® Dye は抗体に共有結合するので、多重染色の際に色素が抗体間を移動したり、拡散したりしません。

本キットは、それぞれ 5-20, 20-50, 50-100 µg の抗体が標識できる3種類のサイズをご用意しており、すべての波長の　CF® Dye　をご使用いただけます。使用する色素量を計算する必要はなく、抗体にキットに含まれる色素を全量混合するだけです。また、標識反応は、バッファーにアジ化物、Tris、BSA、ゼラチン等が含まれていても問題なく進行します。

図1 標識後の精製工程は不要で、わずか30分で抗体を標識できます

特長

簡単なプロトコルで抗体標識が可能
30分で抗体を共有結合で標識
未結合色素の除去不溶
一般的な抗体バッファーに適合

使用例

図2 CF®633 Mix-n-Stain標識したマウス抗ヒトCD3抗体（BD cat# 555330）で染色したJurkat細胞のフローサイトメトリー解析。対照として市販の既存競合蛍光色素標識マウス抗ヒトCD3抗体（BD cat# 557706）で染色した細胞を用いた。蛍光はFL4チャンネルのBD FACSCaliburフローサイトメーターで分析した。

図2 CF633 Mix-n-Stainキットで標識した抗チューブリン抗体
図3 CF633 Mix-n-Stainキットで標識した抗チューブリン抗体（赤）でHela細胞を染色した。アクチンフィラメントはCF488Aファロイジン（緑）で、核はDAPI（青）で染色した。画像はZeiss 510 Meta共焦点顕微鏡で撮影した。

製品一覧

色素名をクリックすると、各色素の詳細をご覧いただけます。

標識	Ex/Em (nm)	標識可能な抗体の量
標識	Ex/Em (nm)	5-20 ug 抗体	20-50 ug 抗体	50-100 ug 抗体
Biotin	N/A	92286	92266	92244
FITC	494/519	92294	92295	92296
CF™350	347/448	92270	92250	92230
CF™405S	404/431	92271	92251	92231
CF™405M	408/452	92272	92252	92232
CF™405L	395/495	92303	92304	92305
CF™430	426/498	92316	92317	92318
CF™440	440/515	92319	92320	92321
CF™450	450/538	92322	92323	92324
CF™488A	490/515	92273	92253	92233
CF™514	516/548	92331	92332	92333
CF™532	527/558	92289	92290	92291
CF™543	541/560	92287	92267	92247
CF™555	555/565	92274	92254	92234
CF™568	562/583	92275	92255	92235
CF™594	593/614	92276	92256	92236
CF™633	630/650	92277	92257	92237
CF™640R	642/662	92278	92258	92238
CF™647	650/665	92279	92259	92239
CF™660C	667/685	92280	92260	92240
CF™660R	663/682	92281	92261	92241
CF™680	681/698	92282	92262	92242
CF™680R	680/701	92283	92263	92243
CF™750	755/777	92284	92264	92245
CF™770	770/797	92285	92265	92246
CF™790	784/806	92288	92268	92248
R-PE	496,564/578	-	92298**	92299
APC	650/660	-	92306**	92307
Per-CP	482/678	-	92308**	92309
APC-CF750T	650/780	-	92310**	92311
DNP	N/A	92325	92326	92327

**25-50 ug

［関連商品］

Mix-n-Stain™ HRP／アルカリホスファターゼ／グルコースオキシダーゼ抗体標識キット

5-20 µg抗体用

品名	メーカー	品番	包装	希望販売価格
Mix-n-Stain^TM Biotin Antibody Labeling Kit, Biotin	BTI	92286	1 KIT [5-20 μg labeling]	￥31,000
Mix-n-Stain^TM FITC Antibody Labeling Kit, Fluorescein Isothiocyanate	BTI	92294	1 KIT	￥19,000
Mix-n-Stain^TM CF^(R)350 Antibody Labeling Kit, CF^TM 350	BTI	92270	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)405S Antibody Labeling Kit, CF^TM 405S	BTI	92271	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)405M Antibody Labeling Kit, CF^TM 405M	BTI	92272	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)405L Antibody Labeling Kit, CF^TM 405L	BTI	92303	1 KIT [1x(5-20ug) labeling]	￥29,000
Mix-n-Stain^TM CF^(R)430 Antibody Labeling Kit, CF^TM	BTI	92316	1 KIT [1x(5-20ug) labeling]	￥36,000
Mix-n-Stain^TM CF^(R)440 Antibody Labeling Kit, CF^TM	BTI	92319	1 KIT [1x(5-20ug) labeling]	￥36,000
Mix-n-Stain^TM CF^(R)450 Antibody Labeling Kit, CF^TM	BTI	92322	1 KIT [1x(5-20ug) labeling]	￥36,000
Mix-n-Stain^TM CF^(R)488A Antibody Labeling Kit, CF^TM 488A	BTI	92273	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)514 Antibody Labeling Kit	BTI	92331	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)532 Antibody Labeling Kit, CF^TM 532	BTI	92289	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)543 Antibody Labeling Kit, CF^TM 543	BTI	92287	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)555 Antibody Labeling Kit, CF^TM 555	BTI	92274	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)568 Antibody Labeling Kit, CF^TM 568	BTI	92275	1 KIT [1x(5-20ug) labeling]	￥29,000
Mix-n-Stain^TM CF^(R)594 Antibody Labeling Kit, CF^TM 594	BTI	92276	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)633 Antibody Labeling Kit, CF^TM 633	BTI	92277	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)640R Antibody Labeling Kit, CF^TM 640R	BTI	92278	1 KIT [1x(5-20ug) labeling]	￥29,000
Mix-n-Stain^TM CF^(R)647 Antibody Labeling Kit, CF^TM 647	BTI	92279	1 KIT [5-20ug labeling]	￥29,000
Mix-n-Stain^TM CF^(R)660C Antibody Labeling Kit, CF^TM 660C	BTI	92280	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)660R Antibody Labeling Kit, CF^TM 660R	BTI	92281	1 KIT [1x(5-20ug) labeling]	￥29,000
Mix-n-Stain^TM CF^(R)680 Antibody Labeling Kit, CF^TM 680	BTI	92282	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)680R Antibody Labeling Kit, CF^TM 680R	BTI	92283	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)750 Antibody Labeling Kit, CF^TM 750	BTI	92284	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)770 Antibody Labeling Kit, CF^TM 770	BTI	92285	1 KIT	￥29,000
Mix-n-Stain^TM CF^(R)790 Antibody Labeling Kit, CF^TM 790	BTI	92288	1 KIT	￥29,000
Mix-n-Stain^TM DNP Antibody Labeling Kit	BTI	92325	1 KIT [1x(5-20ug) labeling]	￥33,000

20-50 µg抗体用

品名	メーカー	品番	包装	希望販売価格
Mix-n-Stain^TM Biotin Antibody Labeling Kit, Biotin	BTI	92266	1 KIT [20-50 μg labeling]	￥33,000
Mix-n-Stain^TM FITC Antibody Labeling Kit, Fluorescein Isothiocyanate	BTI	92295	1 KIT	￥22,000
Mix-n-Stain^TM CF^(R)350 Antibody Labeling Kit, CF^TM 350	BTI	92250	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)405S Antibody Labeling Kit, CF^TM 405S	BTI	92251	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)405M Antibody Labeling Kit, CF^TM 405M	BTI	92252	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)405L Antibody Labeling Kit, CF^TM 405L	BTI	92304	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)430 Antibody Labeling Kit, CF^TM	BTI	92317	1 KIT [1x(20-50ug) labeling]	￥38,000
Mix-n-Stain^TM CF^(R)440 Antibody Labeling Kit, CF^TM	BTI	92320	1 KIT [1x(20-50ug) labeling]	￥38,000
Mix-n-Stain^TM CF^(R)450 Antibody Labeling Kit, CF^TM	BTI	92323	1 KIT [1x(20-50ug) labeling]	￥38,000
Mix-n-Stain^TM CF^(R)488A Antibody Labeling Kit, CF^TM 488A	BTI	92253	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)514 Antibody Labeling Kit	BTI	92332	1 KIT	￥36,000
Mix-n-Stain^TM CF^(R)532 Antibody Labeling Kit, CF^TM 532	BTI	92290	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)543 Antibody Labeling Kit, CF^TM 543	BTI	92267	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)555 Antibody Labeling Kit, CF^TM 555	BTI	92254	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)568 Antibody Labeling Kit, CF^TM 568	BTI	92255	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)594 Antibody Labeling Kit, CF^TM 594	BTI	92256	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)633 Antibody Labeling Kit, CF^TM 633	BTI	92257	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)640R Antibody Labeling Kit, CF^TM 640R	BTI	92258	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)647 Antibody Labeling Kit, CF^TM 647	BTI	92259	1 KIT [20-50ug labeling]	￥32,000
Mix-n-Stain^TM CF^(R)660C Antibody Labeling Kit, CF^TM 660C	BTI	92260	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)660R Antibody Labeling Kit, CF^TM 660R	BTI	92261	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)680 Antibody Labeling Kit, CF^TM 680	BTI	92262	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)680R Antibody Labeling Kit, CF^TM 680R	BTI	92263	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)750 Antibody Labeling Kit, CF^TM 750	BTI	92264	1 KIT [1x(20-50ug) labeling]	￥32,000
Mix-n-Stain^TM CF^(R)770 Antibody Labeling Kit, CF^TM 770	BTI	92265	1 KIT	￥32,000
Mix-n-Stain^TM CF^(R)790 Antibody Labeling Kit, CF^TM 790	BTI	92268	1 KIT	￥32,000
Mix-n-Stain^TM RPE Antibody Labeling Kit, Phycoerythrin -R	BTI	92298	1 KIT [1x(25-50ug) labeling]	販売終了
Mix-n-Stain^TM APC Antibody Labeling Kit, Allophycocyanin	BTI	92306	1 KIT	販売終了
Mix-n-Stain^TM PerCP Antibody Labeling Kit, PerCP	BTI	92308	1 KIT	販売終了
Mix-n-Stain^TM APC-CF^(R)750T Antibody Labeling Kit, CF^TM 750T	BTI	92310	1 KIT	販売終了
Mix-n-Stain^TM DNP Antibody Labeling Kit	BTI	92326	1 KIT [1x(20-50ug) labeling]	￥37,000

50-100 µg抗体用

品名	メーカー	品番	包装	希望販売価格
Mix-n-Stain^TM Biotin Antibody Labeling Kit, Biotin	BTI	92244	1 KIT [50-100μg labeling]	￥37,000
Mix-n-Stain^TM FITC Antibody Labeling Kit, Fluorescein Isothiocyanate	BTI	92296	1 KIT	￥26,000
Mix-n-Stain^TM CF^(R)350 Antibody Labeling Kit, CF^TM 350	BTI	92230	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)405S Antibody Labeling Kit, CF^TM 405S	BTI	92231	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)405M Antibody Labeling Kit, CF^TM 405M	BTI	92232	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)405L Antibody Labeling Kit, CF^TM 405L	BTI	92305	1 KIT [1x(50-100ug) labeling]	￥36,000
Mix-n-Stain^TM CF^(R)430 Antibody Labeling Kit, CF^TM	BTI	92318	1 KIT [1x(50-100ug) labeling]	￥42,000
Mix-n-Stain^TM CF^(R)440 Antibody Labeling Kit, CF^TM	BTI	92321	1 KIT [1x(50-100ug) labeling]	￥42,000
Mix-n-Stain^TM CF^(R)450 Antibody Labeling Kit, CF^TM	BTI	92324	1 KIT [1x(50-100ug) labeling]	￥42,000
Mix-n-Stain^TM CF^(R)488A Antibody Labeling Kit, CF^TM 488A	BTI	92233	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)514 Antibody Labeling Kit	BTI	92333	1 KIT	￥38,000
Mix-n-Stain^TM CF^(R)532 Antibody Labeling Kit, CF^TM 532	BTI	92291	1 KIT	￥36,000
Mix-n-Stain^TM CF^(R)543 Antibody Labeling Kit, CF^TM 543	BTI	92247	1 KIT	￥36,000
Mix-n-Stain^TM CF^(R)555 Antibody Labeling Kit, CF^TM 555	BTI	92234	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)568 Antibody Labeling Kit, CF^TM 568	BTI	92235	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)594 Antibody Labeling Kit, CF^TM 594	BTI	92236	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)633 Antibody Labeling Kit, CF^TM 633	BTI	92237	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)640R Antibody Labeling Kit, CF^TM 640R	BTI	92245	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)647 Antibody Labeling Kit, CF^TM 647	BTI	92238	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)660C Antibody Labeling Kit, CF^TM 660C	BTI	92239	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)660R Antibody Labeling Kit, CF^TM 660R	BTI	92243	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)680 Antibody Labeling Kit, CF^TM 680	BTI	92240	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)680R Antibody Labeling Kit, CF^TM 680R	BTI	92246	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)750 Antibody Labeling Kit, CF^TM 750	BTI	92241	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)770 Antibody Labeling Kit, CF^TM 770	BTI	92242	1 KIT [1x(50-100μg)labeling]	￥36,000
Mix-n-Stain^TM CF^(R)790 Antibody Labeling Kit, CF^TM 790	BTI	92248	1 KIT	￥36,000
Mix-n-Stain^TM RPE Antibody Labeling Kit, Phycoerythrin -R	BTI	92299	1 KIT [1x(50-100ug) labeling]	販売終了
Mix-n-Stain^TM APC Antibody Labeling Kit, Allophycocyanin	BTI	92307	1 KIT	販売終了
Mix-n-Stain^TM PerCP Antibody Labeling Kit, PerCP	BTI	92309	1 KIT	販売終了
Mix-n-Stain^TM APC-CF^(R)750T Antibody Labeling Kit, CF^TM 750T	BTI	92311	1 KIT	販売終了
Mix-n-Stain^TM DNP Antibody Labeling Kit	BTI	92327	1 KIT [1x(50-100ug) labeling]	￥40,000